Extended interaction network of procollagen C-proteinase enhancer-1 in the extracellular matrix

Romain Salza, Franck Peysselon, Emilie Chautard, Clément Faye, Laura Moschcovich, Tali Weiss, Laure Perrin-Cocon, Vincent Lotteau, Efrat Kessler, Sylvie Ricard-Blum*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


PCPE-1 (procollagen C-proteinase enhancer-1) is an extracellular matrix glycoprotein that can stimulate procollagen processing by procollagen C-proteinases such as BMP-1 (bone morphogenetic protein 1). PCPE-1 interacts with several proteins in addition to procollagens and BMP-1, suggesting that it could be involved in biological processes other than collagen maturation. We thus searched for additional partners of PCPE-1 in the extracellular matrix, which could provide new insights into its biological roles. We identified 17 new partners of PCPE-1 by SPR (surface plasmon resonance) imaging. PCPE-1 forms a transient complex with the β-amyloid peptide, whereas it forms high or very high affinity complexes with laminin-111 (KD =58.8 pM), collagen VI (KD =9.5 nM), TSP-1 (thrombospondin-1) (KD1 =19.9 pM, KD2 =14.5 nM), collagen IV (KD =49.4 nM) and endostatin, a fragment of collagen XVIII (KD1 =0.30 nM, KD2 =1.1 nM). Endostatin binds to the NTR (netrin-like) domain of PCPE-1 and decreases the degree of superstimulation of PCPE-1 enhancing activity by heparin. The analysis of the PCPE-1 interaction network based on Gene Ontology terms suggests that, besides its role in collagen deposition, PCPE-1 might be involved in tumour growth, neurodegenerative diseases and angiogenesis. In vitro assays have indeed shown that the CUB1CUB2 (where CUB is complement protein subcomponents C1r/C1s, urchin embryonic growth factor and BMP-1) fragment of PCPE-1 inhibits angiogenesis. The Authors Journal compilation

Original languageEnglish
Pages (from-to)137-149
Number of pages13
JournalBiochemical Journal
Issue number1
StatePublished - 1 Jan 2014


FundersFunder number
European CommissionNMP2-CT-2003-504017
Seventh Framework Programme223411


    • Affinity
    • Extracellular matrix
    • Kinetics
    • Network
    • Protein-protein interaction
    • Surface plasmon resonance


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