TY - JOUR
T1 - Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7)
AU - Dessau, Moshe
AU - Chamovitz, Daniel A.
AU - Hirsch, Joel A.
PY - 2006/11
Y1 - 2006/11
N2 - A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using polyethylene glycol 8000 as a precipitant and have a thick rod-like morphology. Their crystallographic symmetry is orthorhombic, space group C2221, with unit-cell parameters a = 57.2, b = 86.2, c = 72.6 Å and a diffraction limit of 2.06 Å.
AB - A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using polyethylene glycol 8000 as a precipitant and have a thick rod-like morphology. Their crystallographic symmetry is orthorhombic, space group C2221, with unit-cell parameters a = 57.2, b = 86.2, c = 72.6 Å and a diffraction limit of 2.06 Å.
KW - COP9 signalosome
KW - PCI and MPN motifs
KW - Proteasome
UR - http://www.scopus.com/inward/record.url?scp=33750584549&partnerID=8YFLogxK
U2 - 10.1107/S1744309106041959
DO - 10.1107/S1744309106041959
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AN - SCOPUS:33750584549
SN - 1744-3091
VL - 62
SP - 1138
EP - 1140
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 11
ER -