Expression of the leptin receptor in different types of vascular lesions

Marco R. Schroeter, Jacob Schneiderman, Bettina Schumann, Roland Glückermann, Petros Grimmas, Arnd B. Buchwald, Theodor Tirilomis, Friedrich A. Schöndube, Stavros V. Konstantinides, Katrin Schäfer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Clinical and experimental evidence suggests that the adipokine leptin may be important for the development of cardiovascular complications associated with obesity, possibly through interaction with its receptor on vascular cells. In the present study, we systematically analysed expression of the leptin receptor in normal and diseased vascular specimens using immunohistochemistry, immunofluorescence and quantitative real time-PCR. In particular, human atherosclerotic plaques as well as experimental vascular lesions induced in hypercholesterolemic mice and minipigs, respectively, were examined. Our results demonstrate the presence of the leptin receptor in normal vessel wall segments as well as neointimal or atherosclerotic lesions. In the latter, ObR expressing cells were predominantly localised on the luminal border and within the subintima, and coexpression of von Willebrand factor, VEGF receptor-2 or VE cadherin identified them as endothelial cells. Moreover, CD14-positive monocytes/macrophages were strongly positive for the leptin receptor. In contrast, only few ObR-expressing smooth muscle cells could be detected in human atherosclerotic plaques. The findings of the present study thus support a possible action of leptin on the cardiovascular system by demonstrating expression of the leptin receptor in different types of vascular lesions.

Original languageEnglish
Pages (from-to)323-333
Number of pages11
JournalHistochemistry and Cell Biology
Volume128
Issue number4
DOIs
StatePublished - Oct 2007

Funding

FundersFunder number
Novartis Stiftung

    Keywords

    • Atherosclerosis
    • Fluorescence microscopy
    • Leptin receptor
    • Neointima
    • Obesity

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