TY - JOUR
T1 - Expression of stem cell factor and its receptor in human fetal and adult ovaries
AU - Abir, Ronit
AU - Fisch, Benjamin
AU - Jin, Shaoguang
AU - Barnnet, Mirian
AU - Kessler-Icekson, Gania
AU - Ao, Asangla
N1 - Funding Information:
Supported in part by research grants from the Brazilian Friends of the Israel Cancer Association and Tel Aviv University (Leo Mintz Fund).
PY - 2004/10
Y1 - 2004/10
N2 - To investigate the immunocytochemical expression and presence of mRNA transcripts of stem cell factor (SCF) and its receptor (SCF-R) in ovaries from human adults and fetuses. Immunocytochemical and reverse transcription polymerase chain reaction (RT-PCR) study. Major tertiary care and referral academic centers. Seven women and girls undergoing laparoscopic ovarian biopsy and 13 women undergoing second and third trimester pregnancy terminations. None. Microscopic morphometric analysis, immunocytochemistry for SCF and SCF-R, and RT-PCR analyses. There was strong to moderate immunocytochemical staining for SCF and its receptor in oocytes from primordial stages onward, but not in granulosa cells, in both fetal and adult ovarian samples. Transcripts of SCF and SCF-R RNA were detected by RT-PCR analyses for SCF and SCF-R. The expression of SCF and its receptor in ovarian tissue from fetuses and women suggests a possible role of SCF in growth initiation of human primordial follicles.
AB - To investigate the immunocytochemical expression and presence of mRNA transcripts of stem cell factor (SCF) and its receptor (SCF-R) in ovaries from human adults and fetuses. Immunocytochemical and reverse transcription polymerase chain reaction (RT-PCR) study. Major tertiary care and referral academic centers. Seven women and girls undergoing laparoscopic ovarian biopsy and 13 women undergoing second and third trimester pregnancy terminations. None. Microscopic morphometric analysis, immunocytochemistry for SCF and SCF-R, and RT-PCR analyses. There was strong to moderate immunocytochemical staining for SCF and its receptor in oocytes from primordial stages onward, but not in granulosa cells, in both fetal and adult ovarian samples. Transcripts of SCF and SCF-R RNA were detected by RT-PCR analyses for SCF and SCF-R. The expression of SCF and its receptor in ovarian tissue from fetuses and women suggests a possible role of SCF in growth initiation of human primordial follicles.
KW - Primordial follicles
KW - SCF
KW - SCF-R
KW - immunocytochemistry
KW - polymerase chain reaction
KW - reverse transcription
UR - http://www.scopus.com/inward/record.url?scp=5044238924&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2004.02.146
DO - 10.1016/j.fertnstert.2004.02.146
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AN - SCOPUS:5044238924
SN - 0015-0282
VL - 82
SP - 1235
EP - 1243
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - SUPPL. 3
ER -