@article{282223b5ddd7411fa37f13a78bde4952,
title = "Expression of small heat shock protein αB-crystallin is induced after hepatic stellate cell activation",
abstract = "Using the differential PCR display method to select cDNA fragments that are differentially expressed after hepatic stellate cell (HSC) activation, we have isolated from activated HSCs a cDNA that corresponds to rat αB-crystallin. Northern blots confirmed expression of αB-crystallin in culture-activated HSCs but not in quiescent HSCs. Western blot analysis and immunocytochemical staining confirmed expression of αB-crystallin protein in activated but not quiescent HSCs. αB-crystallin is induced as early as 6 h after plating HSCs on plastic and continues to be expressed for 14 days in culture. Expression of αB-crystallin was also induced in vivo in activated HSCs from experimental cholestatic liver fibrosis. Confocal microscopy demonstrated a cytoplasmic distribution of αB-crystallin in a cytoskeletal pattern. Heat shock treatment resulted in an immediate perinuclear redistribution that in time returned to a normal cytoskeletal distribution. The expression pattern of αB-crystallin was similar to that of HSP25, another small heat shock protein, but differed from the classic heat shock protein HSP70. Therefore, αB-crystallin represents an early marker for HSC activation.",
keywords = "Differential polymerase chain reaction display, Gene expression, Liver fibrosis",
author = "Alon Lang and Schrum, {Laura W.} and Robert Schoonhoven and Shmuel Tuvia and Sol{\'i}s-Herruzo, {Jose A.} and Hidekazu Tsukamoto and Brenner, {David A.} and Rippe, {Richard A.}",
year = "2000",
doi = "10.1152/ajpgi.2000.279.6.g1333",
language = "אנגלית",
volume = "279",
pages = "G1333--G1342",
journal = "American Journal of Physiology - Gastrointestinal and Liver Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "6 42-6",
}