Expression of SDF-1/CXCR4 in injured human kidneys

Danny Lotan, Natalia Sheinberg, Jury Kopolovic, Benjamin Dekel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The chemokine SDF-1α is involved in migration, survival, and development of multiple cells, most notably of hematopoietic stem cells (HSC) expressing its ligand CXCR4. Recently, we have shown engraftment of human HSC in the ischemically injured murine kidney, presumably mediated by SDF-1α. To further investigate a possible role of SDF-1α in the recruitment of CXCR4+ cells in human renal disease of varying etiologies, we immunostained human biopsies of immunoglobulin (Ig)A nephropathy, minimal-change nephrotic syndrome, focal segmental glomerulosclerosis, membranoproliferative glomerulonephritis, chronic pyelonephritis, and acute tubular necrosis (ATN) for SDF-1α, CXCR4, and CD45, a pan-hematopoietic marker. Irrespective of the diagnosis, intense SDF-1α immunoreactivity was localized to distal tubules and collecting ducts, whereas CXCR4 showed intense staining in both distal and proximal tubules. In addition, whereas varying degrees of CD45+ cell infiltrates were observed in all biopsies, we found focal infiltrates of CXCR4+ cells mostly localized to the corticomedullary junction only in ischemic ATN. This correlated with more extensive staining for SDF-1α in these sites. In all investigated renopathologic conditions, CD45+ leukocyte recruitment to the kidney seems not to be driven by SDF-1α/CXCR4 interaction. A contribution of SDF-1α for influx of CXCR4+ cells in the vicinity of arcuate vessels is suggested only in human ATN.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalPediatric Nephrology
Volume23
Issue number1
DOIs
StatePublished - Jan 2008

Funding

FundersFunder number
Israel Science Foundation

    Keywords

    • CD45
    • Ischemic injury
    • Renal inflammation
    • SDF-1α/CXCR4
    • Stem cells

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