TY - JOUR
T1 - Expression of mannose-ligand receptors on human spermatozoa
T2 - Effect of lecithin and association with sperm binding to the zona pellucida
AU - Gamzu, Ronni
AU - Yogev, Leah
AU - Kleiman, Sandra
AU - Botchan, Amnon
AU - Hauser, Ron
AU - Lessing, Joseph B.
AU - Paz, Gedalia
AU - Yavetz, Haim
N1 - Funding Information:
Supported by the Leo Mintz Fund for Gynecological Research, Tel Aviv, Israel.
PY - 1998/10
Y1 - 1998/10
N2 - Objective: To evaluate the change in the expression of mannose-ligand receptors and sperm binding capacity after the incubation of sperm cells with lecithin liposomes. Design: A randomized, blinded-controlled experiment. Setting: Andrology laboratory at the Lis Maternity Hospital. Patient(s): Fifteen fertile sperm donors and 10 subfertile men. Intervention(s): Incubation of sperm samples with either control medium or 1 mg/mL of liposomal lecithin for 2 hours. Main Outcome Measure(s): Expression of mannose-ligand receptors as evaluated by mannosylated bovine serum albumin- fluorescein isothiocyanate and sperm binding to the zona pellucida as evaluated by the hemizona assay. Result(s): The mean ± SE percentages of spermatozoa with patterns I, II, and III were 86% ± 4.8%, 11% ± 3.4%, and 3% ± 1.6%, respectively, after treatment with control medium and 71% ± 5.7%, 22% ± 3.5%, and 7% ± 2.5%, respectively, after treatment with lecithin. The same effect of lecithin was observed in the 10 sperm samples from subfertile men. The mean ± SE numbers of sperm that bound to hemizonae after treatment with control medium or lecithin were 116 ± 32.4 and 176 ± 29.6, respectively. Statistically significant correlations were observed between the shift in patterns II and III and the enhancement of sperm binding after lecithin treatment (r = 0.44 and 0.6, respectively). Conclusion(s): Lecithin shifts the expression of mannose-ligand receptors to the capacitated and acrosome-reacted patterns and enhances the binding capacity of the sperm cells.
AB - Objective: To evaluate the change in the expression of mannose-ligand receptors and sperm binding capacity after the incubation of sperm cells with lecithin liposomes. Design: A randomized, blinded-controlled experiment. Setting: Andrology laboratory at the Lis Maternity Hospital. Patient(s): Fifteen fertile sperm donors and 10 subfertile men. Intervention(s): Incubation of sperm samples with either control medium or 1 mg/mL of liposomal lecithin for 2 hours. Main Outcome Measure(s): Expression of mannose-ligand receptors as evaluated by mannosylated bovine serum albumin- fluorescein isothiocyanate and sperm binding to the zona pellucida as evaluated by the hemizona assay. Result(s): The mean ± SE percentages of spermatozoa with patterns I, II, and III were 86% ± 4.8%, 11% ± 3.4%, and 3% ± 1.6%, respectively, after treatment with control medium and 71% ± 5.7%, 22% ± 3.5%, and 7% ± 2.5%, respectively, after treatment with lecithin. The same effect of lecithin was observed in the 10 sperm samples from subfertile men. The mean ± SE numbers of sperm that bound to hemizonae after treatment with control medium or lecithin were 116 ± 32.4 and 176 ± 29.6, respectively. Statistically significant correlations were observed between the shift in patterns II and III and the enhancement of sperm binding after lecithin treatment (r = 0.44 and 0.6, respectively). Conclusion(s): Lecithin shifts the expression of mannose-ligand receptors to the capacitated and acrosome-reacted patterns and enhances the binding capacity of the sperm cells.
KW - Lecithin
KW - Mannose-ligand receptors
KW - Spermatozoa
UR - http://www.scopus.com/inward/record.url?scp=0031754556&partnerID=8YFLogxK
U2 - 10.1016/S0015-0282(98)00262-3
DO - 10.1016/S0015-0282(98)00262-3
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AN - SCOPUS:0031754556
SN - 0015-0282
VL - 70
SP - 766
EP - 770
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 4
ER -