Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice

Rakesh K. Singh; Mordechai Gutman; Robert Radinsky; Corazon D. Bucana; Isaiah J. Fidler

Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice

Rakesh K. Singh, Mordechai Gutman, Robert Radinsky, Corazon D. Bucana, Isaiah J. Fidler

Research output: Contribution to journal › Article › peer-review

Abstract

We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.

Original language	English
Pages (from-to)	3242-3247
Number of pages	6
Journal	Cancer Research
Volume	54
Issue number	12
State	Published - 15 Jun 1994
Externally published	Yes

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title = "Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice",

abstract = "We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.",

author = "Singh, {Rakesh K.} and Mordechai Gutman and Robert Radinsky and Bucana, {Corazon D.} and Fidler, {Isaiah J.}",

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AU - Singh, Rakesh K.

AU - Gutman, Mordechai

AU - Radinsky, Robert

AU - Bucana, Corazon D.

AU - Fidler, Isaiah J.

PY - 1994/6/15

Y1 - 1994/6/15

N2 - We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.

AB - We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.

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Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice

Abstract

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