Expression of inducible nitric oxide synthase in a mouse model of anaphylaxis

Kobi Sade*, I. F. Schwartz, S. Etkin, S. Schwartzenberg, Y. Levo, S. Kivity

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Background: The generation of large quantities of nitric oxide (NO) is implicated in the pathogenesis of anaphylactic shock. The source of NO, however, has not been established and conflicting results have been obtained when investigators have tried to inhibit its production in anaphylaxis. Objective: The aim of this study was to analyze the expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in a mouse model of anaphylaxis. Methods: BALB/c mice were sensitized and challenged with ovalbumin to induce anaphylaxis. Tissues were removed from the heart and lungs, and blood was drawn at different time points during the first 48 hours after induction of anaphylaxis. The Griess assay was used to measure nitric oxide generation. Nitric oxide synthase expression was examined by reverse transcriptase polymerase chain reaction and immunohistochemistry. Results: A significant increase in iNOS mRNA expression and nitric oxide production was evident as early as 10 to 30 minutes after allergen challenge in both heart and lungs. In contrast, expression of eNOS mRNA was not altered during the course of the experiment. Conclusion: Our results support involvement of iNOS in the immediate physiological response of anaphylaxis.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalJournal of Investigational Allergology and Clinical Immunology
Issue number6
StatePublished - 2007


  • Allergy
  • Anaphylaxis
  • Inducible nitric oxide synthase
  • Nitric oxide
  • Shock


Dive into the research topics of 'Expression of inducible nitric oxide synthase in a mouse model of anaphylaxis'. Together they form a unique fingerprint.

Cite this