Expression of inducible nitric oxide synthase by neurones following exposure to endotoxin and cytokine

Dahlia Minc‐Golomb, Ilan Tsarfaty, Joan P. Schwartz

Research output: Contribution to journalArticlepeer-review

Abstract

In the CNS, nitric oxide (NO) has been implicated as both a mediator of neurotoxicity and a neuromodulator. The inducible NO synthase (iNOS), thought to mediate toxic effects of NO, has been attributed to glial cells in the CNS. We now report that cerebellar granule cell neurones can be stimulated by lipopolysaccharide and interferon‐γ to express iNOS in vitro, as demonstrated by reverse transcription‐polymerase chain reaction and fluorescent in situ hybridisation. The expression of both constitutive NO synthase (cNOS) and iNOS by neurones suggests that NO has diverse functions in the brain, and supports the possibility that iNOS plays a role in neuronal damage and inflammation following activation of brain microglia and production of cytokines. 1994 British Pharmacological Society

Original languageEnglish
Pages (from-to)720-722
Number of pages3
JournalBritish Journal of Pharmacology
Volume112
Issue number3
DOIs
StatePublished - Jul 1994
Externally publishedYes

Keywords

  • Cerebellar granule cells
  • cerebellum
  • interferon‐γ
  • lipopolysaccharide
  • macrophage

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