TY - JOUR
T1 - Expression of growth-differentiating factor 9 and its type 1 receptor in human ovaries
AU - Oron, Galia
AU - Fisch, Benjamin
AU - Ao, Asangla
AU - Zhang, Xiao Yun
AU - Farhi, Jacob
AU - Haroush, Avi Ben
AU - Kesseler-Icekson, Gania
AU - Abir, Ronit
N1 - Funding Information:
The authors are greatly indebted to Carmela Felz, laboratory technician, for preparation of the histological sections. The authors are grateful to Ms Gloria Ganzach from the Editorial Board for the English editing, to the staff at the Gynecology Ward for their help in locating suitable patients and to the Ultrasound Unit for identifying fetal gender (all from Rabin Medical Centre, Beilinson Hospital). The study was partially funded by a research grant from the Israel Cancer Association.
PY - 2010/7
Y1 - 2010/7
N2 - The expression of growth-differentiating factor 9 (GDF9) has not been studied in ovaries from girls and human fetuses nor has its receptor transforming growth factor-β1 receptor (TGFβR1) been investigated in ovaries of girls/women. The aim of this study was to fill these gaps. Ovarian samples were obtained from 16 human fetuses at 21-35 gestational weeks and from 34 girls/women aged 5-39 years. These specimens were prepared for immunohistochemical staining of the GDF9 and TGFβR1 proteins. Reverse transcription polymerase chain reaction was used to detect GDF9 mRNA transcripts and in-situ hybridization to localize TGFβR1 mRNA transcripts. Positive staining for the GDF9 protein was identified in oocytes and granulosa cells in all samples tested. GDF9 mRNA transcripts were present in all samples. Protein expression of TGFβR1 was identified in granulosa cells in all samples. Oocyte staining was identified in samples from girls/women but in only one fetal sample. TGFβR1 mRNA transcripts were identified in granulosa cells and oocytes in 50% of the samples from fetuses aged over 22 gestational weeks and in samples from girls/women. The detection of GDF9 and TGFβR1 at both at the protein and mRNA levels suggests that GDF9 may have functions in human preantral follicles.
AB - The expression of growth-differentiating factor 9 (GDF9) has not been studied in ovaries from girls and human fetuses nor has its receptor transforming growth factor-β1 receptor (TGFβR1) been investigated in ovaries of girls/women. The aim of this study was to fill these gaps. Ovarian samples were obtained from 16 human fetuses at 21-35 gestational weeks and from 34 girls/women aged 5-39 years. These specimens were prepared for immunohistochemical staining of the GDF9 and TGFβR1 proteins. Reverse transcription polymerase chain reaction was used to detect GDF9 mRNA transcripts and in-situ hybridization to localize TGFβR1 mRNA transcripts. Positive staining for the GDF9 protein was identified in oocytes and granulosa cells in all samples tested. GDF9 mRNA transcripts were present in all samples. Protein expression of TGFβR1 was identified in granulosa cells in all samples. Oocyte staining was identified in samples from girls/women but in only one fetal sample. TGFβR1 mRNA transcripts were identified in granulosa cells and oocytes in 50% of the samples from fetuses aged over 22 gestational weeks and in samples from girls/women. The detection of GDF9 and TGFβR1 at both at the protein and mRNA levels suggests that GDF9 may have functions in human preantral follicles.
KW - GDF9
KW - TGFβR1
KW - human ovaries
KW - immunohistochemistry
KW - in-situ hybridization
KW - reverse transcription polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=78650126137&partnerID=8YFLogxK
U2 - 10.1016/j.rbmo.2010.03.011
DO - 10.1016/j.rbmo.2010.03.011
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AN - SCOPUS:78650126137
SN - 1472-6483
VL - 21
SP - 109
EP - 117
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
IS - 1
ER -
