Expression of CDY1 may identify complete spermatogenesis

Sandra E. Kleiman*, Ayala Lagziel, Leah Yogev, Amnon Botchan, Gedalia Paz, Haim Yavetz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Objective: To investigate the expression of deleted in azoospermia (DAZ), RNA-binding motif (RBM), and chromodomain y1 (CDY1) genes in the testes of men with azoospermia with variable histopathologies. Design: Prospective study. Setting: Andrology laboratory of a university-affiliated maternity hospital. Patient(s): Sixty-six men with azoospermia. Intervention(s): Testicular sperm extraction. Main Outcome Measure(s): The results of gene expression in testicular tissue tested by RT-PCR were correlated with those of histopathologically and microscopically examined minced testicular tissue. Y chromosome microdeletion testing and karyotyping were performed, as was direct sequencing of CDY1-PCR products. Result(s): CDY1-minor expression was detected in all biopsies in which mature spermatids/spermatozoa were observed by histological analysis and/or in the minced tissue. CDY1-minor expression was also detected in two biopsies with arrest at the spermatocyte stage during which no mature spermatids/spermatozoa were observed. A previously unreported CDY1-minor alternative splicing transcript was identified. DAZ and RBM gene expressions were detected in all biopsies in which at least a few germinal cells in early stages were found and in one biopsy histologically determined as Sertoli cell only. Conclusion(s): Our preliminary results suggest that CDY1-minor expression might increase the prospect for complete spermatogenesis, while RBM and DAZ expression can only be indicative of the presence of germinal cells.

Original languageEnglish
Pages (from-to)166-173
Number of pages8
JournalFertility and Sterility
Issue number1
StatePublished - 2001
Externally publishedYes


  • AZF gene expression
  • Azoospermia
  • CDY1
  • Germinal cells
  • Spermatogenesis


Dive into the research topics of 'Expression of CDY1 may identify complete spermatogenesis'. Together they form a unique fingerprint.

Cite this