TY - JOUR
T1 - Expression of basic fibroblast growth factor and its receptors in human ovarian follicles from adults and fetuses
AU - Ben-Haroush, Avi
AU - Abir, Ronit
AU - Ao, Asangla
AU - Jin, Shaoguang
AU - Kessler-Icekson, Gania
AU - Feldberg, Dov
AU - Fisch, Benjamin
N1 - Funding Information:
This project was partially sponsored by the Israel Cancer Association (R.A., B.F.) and by a research grant from the Steering Committee for Research Promotion at Rabin Medical Center (A.B.H.).
PY - 2005/10
Y1 - 2005/10
N2 - Objective: To investigate the immunocytochemical expression and presence of mRNA transcripts for basic fibroblast growth factor (bFGF) and its four receptors (FGFR-1, -2, -3, and -4) in ovaries from human adults and fetuses. Design: Immunocytochemical and reverse transcription polymerase chain reaction (RT-PCR) study. Setting: Major tertiary care and referral academic centers. Patient(s): Nine women and girls undergoing laparoscopic ovarian biopsy and 26 women undergoing second- and third-trimester pregnancy terminations. Intervention(s): None. Main Outcome Measure(s): Microscopic morphometric analysis, immunocytochemistry for bFGF and its receptors, and RT-PCR analyses. Result(s): The proteins for bFGF, FGFR-2, FGFR-3, and FGFR-4 were identified in oocytes of all follicular classes. Immunocytochemical expression of bFGF and its receptors was detected in granulosa cells of follicles from adolescents/women but not from fetuses. There was no immunocytochemical expression of FGFR-1. Transcripts of bFGF and its four receptors were identified by RT-PCR in all samples. Conclusion(s): The expression of bFGF and its receptors in human ovaries suggests that bFGF might have a role in early folliculogenesis.
AB - Objective: To investigate the immunocytochemical expression and presence of mRNA transcripts for basic fibroblast growth factor (bFGF) and its four receptors (FGFR-1, -2, -3, and -4) in ovaries from human adults and fetuses. Design: Immunocytochemical and reverse transcription polymerase chain reaction (RT-PCR) study. Setting: Major tertiary care and referral academic centers. Patient(s): Nine women and girls undergoing laparoscopic ovarian biopsy and 26 women undergoing second- and third-trimester pregnancy terminations. Intervention(s): None. Main Outcome Measure(s): Microscopic morphometric analysis, immunocytochemistry for bFGF and its receptors, and RT-PCR analyses. Result(s): The proteins for bFGF, FGFR-2, FGFR-3, and FGFR-4 were identified in oocytes of all follicular classes. Immunocytochemical expression of bFGF and its receptors was detected in granulosa cells of follicles from adolescents/women but not from fetuses. There was no immunocytochemical expression of FGFR-1. Transcripts of bFGF and its four receptors were identified by RT-PCR in all samples. Conclusion(s): The expression of bFGF and its receptors in human ovaries suggests that bFGF might have a role in early folliculogenesis.
KW - Immunocytochemistry
KW - Primordial follicles
KW - RT-PCR
KW - bFGF
KW - bFGF receptors
KW - mRNA
UR - http://www.scopus.com/inward/record.url?scp=25844527438&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2005.05.018
DO - 10.1016/j.fertnstert.2005.05.018
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C2 - 16210019
AN - SCOPUS:25844527438
SN - 0015-0282
VL - 84
SP - 1257
EP - 1268
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - SUPPL. 2
ER -