Expression of angiogenesis-related genes in ovarian carcinoma - A clinicopathologic study

B. Davidson, I. Goldberg, J. Kopolovic, W. H. Gotlieb, V. Givant-Horwitz, J. M. Nesland, A. Berner, G. Ben-Baruch, M. Bryne, R. Reich

Research output: Contribution to journalArticlepeer-review

Abstract

Angiogenic factors play a role in tumor growth and spread. The object of this study was to analyze the correlation between mRNA expression of angiogenesis-related genes and disease outcome in advanced-stage ovarian carcinomas. Sections from 66 primary ovarian carcinomas and metastatic lesions from 41 patients diagnosed with advanced stage ovarian carcinoma (FIGO stages III-IV) were evaluated for expression of basic fibroblast factor (bFGF), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) using mRNA In Situ Hybridization (ISH). Patients were divided in two groups based on disease outcome. Long-term survivors (17 patients) and short-term survivors (24 patients) were defined using a double cut-off of 36 months for disease-free survival (DFS) and 60 months for overall survival (OS). Mean follow-up period was 70 months. The mean values for DFS and OS were 116 and 133 months for long-term survivors, as compared to 3 and 21 months for short-term survivors, respectively. Expression of bFGF mRNA, most often intense, was detected in tumor and stromal cells in the majority of cases. Weak expression of IL-8 mRNA was detected in both cell compartments, while VEGF mRNA expression was limited to few cases. Primary tumors displayed higher bFGF and IL-8 mRNA expression. However, these differences did not reach statistical significance (P > 0.05). bFGF, IL-8 and VEGF mRNA expression in both tumor and stromal cells was comparable in tumors of long-term and short-term survivors, and showed no correlation with disease outcome in survival analysis (P > 0.05). bFGF is the major angiogenic factor expressed in ovarian carcinoma at the mRNA level, mRNA expression of VEGF, bFGF, and IL-8 does not appear to be a predictor of disease outcome in advanced-stage ovarian carcinoma.

Original languageEnglish
Pages (from-to)501-508
Number of pages8
JournalClinical and Experimental Metastasis
Volume18
Issue number6
DOIs
StatePublished - 2000

Keywords

  • Basic fibroblast factor
  • Disease outcome
  • Interleukin-8
  • MRNA in situ hybridization
  • Ovarian carcinoma
  • Vascular endothelial growth factor

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