@article{dee99ab6fa294fdeb4c61c555be9d028,
title = "Expression of Acetylcholine Receptor Subunits in Saccharomyces cerevisiae (Yeast)",
abstract = "The chapter presents yeast as an expression system for neuronal receptors: to use yeast to amplify the amounts of receptor proteins that normally exist in only small quantities in cells so that their structure and function can be studied. Because yeast can easily be grown in large quantities, it has obvious advantages over oocytes—an expression system in which each cell has to be injected to obtain relatively little protein. Therefore, with the development of the technology to produce large enough quantities of membrane proteins in yeast to investigate their structure and function, yeast cells can become a good source for the approximately 20 neuronal receptors that exist in only small quantities in cells and about which relatively little is known.",
author = "Sweet, {Melody T.} and Kathrin Jansen and Min, {Churl K.} and Hess, {George P.} and Norihisa Fujita and Fox, {Thomas D.} and Jon Lindstrom and Toni Claudio and Nathan Nelson",
note = "Funding Information: Supported by a grant from the Cornell Biotechnology Program, which is supported by the New York State Science and Technology Foundation and a consortium of industries. We thank Ben Hall for the yeast expression vector pMAC561. M. T. S. was supported by a Muscular Dystrophy Association Postdoctoral Fellowship, K. J. by a Feodor Lynen Postdoctoral Fellowship awarded by the Alexander von Humboldt Foundation, and C. K. M. by a scholarship from the Korean Ministry of Education.",
year = "1988",
month = jan,
doi = "10.1016/S0070-2161(08)60900-4",
language = "אנגלית",
volume = "33",
pages = "197--218",
journal = "Current Topics in Membranes and Transport",
issn = "0070-2161",
publisher = "Academic Press Inc.",
number = "C",
}