@article{aa35149b06b142c781dd06b44da8b1ae,
title = "Expression levels of RGS7 and RGS4 proteins determine the mode of regulation of the G protein-activated K+ channel and control regulation of RGS7 by Gβ5",
abstract = "Regulators of G protein signaling RGS4 and RGS7 accelerate the kinetics of K+ channels (GIRKs) in the Xenopus oocyte system. Here, via quantitative analysis of RGS expression, we reveal biphasic effects of RGSs on GIRK regulation. At low concentrations, RGS4 inhibited basal GIRK activity, but stimulated it at high concentrations. RGS7, which is associated with the G protein subunit Gβ5, is regulated by Gβ5 by two distinct mechanisms. First, Gβ5 augments RGS7 activity, and second, it increases its expression. These dual effects resolve previous controversies regarding RGS4 and RGS7 function and indicate that they modulate signaling by mechanisms supplementary to their GTPase-activating protein activity.",
keywords = "G protein, G protein-activated K channel, Gβ5, Potassium channel, Regulator of G protein signaling, Xenopus oocyte",
author = "Tal Keren-Raifman and Bera, {Amal K.} and Dror Zveig and Sagit Peleg and Witherow, {D. Scott} and Slepak, {Vladlen Z.} and Nathan Dascal",
note = "Funding Information: This study was supported by NIH Grants R01 GM56260 (N.D.), GM60019 (V.Z.S.), USA–Israel Binational Science Foundation (N.D.), a predoctoral fellowship from the American Heart Association (D.S.W.). ",
year = "2001",
month = mar,
day = "9",
doi = "10.1016/S0014-5793(01)02220-7",
language = "אנגלית",
volume = "492",
pages = "20--28",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "1-2",
}