@article{6392e9a1bccb43ef8d1ac73e45070fbf,
title = "Expression cloning of KCRF, a potassium channel regulatory factor",
abstract = "By functional coexpression screening of a rat cDNA library in Xenopus oocytes, we have cloned a protein (KCRF: K Channel Regulatory Factor) that reduces currents of several K+ channels: G protein-activated GIRK1/4 (K(ir)3.1/K(ir)3.4), inward rectifier IRK1 (K(ir)2.1), and voltage-dependent K(V)1.1/K(V)β1.1. KCRF did not modulate two other K+ channels: ROMK1 (K(ir)1.1) and GIRK1/2 (K(ir)3.1/K(ir)3.2) and the voltage-dependent L-type Ca2+ channels. Western blot analysis showed that KCRF is ubiquitous in rat tissues. Biochemical and electrophysiological experiments revealed that coexpression of KCRF causes a decrease in the level of expression of IRK1 and K(V)1.1/K(V)β1.1 proteins in the oocytes. (C) 2000 Academic Press.",
keywords = "Calcium channel, G protein, Ion channel, Protein biosynthesis, RNA splicing, Xenopus oocyte",
author = "Tal Keren-Raifman and Tatiana Ivanina and Yona Bismuth and Nathan Dascal",
note = "Funding Information: We thank Ilana Lotan for critical reading of the manuscript, Rachel Barzilai for subcloning of IRK1 into the pGEM-HJ vector, and Yehudit Zipser for advice. This work was supported by grants from NIH (GM 56260) and the USA-Israel Binational Science Foundation (96-201).",
year = "2000",
month = aug,
day = "11",
doi = "10.1006/bbrc.2000.3240",
language = "אנגלית",
volume = "274",
pages = "852--858",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",
}