Expression and characterization of the integrase of bovine immunodeficiency virus

Orna Avidan, Amnon Hizi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In the pol gene of bovine immunodeficiency virus (BIV) there is a sequence, located between the reverse-transcriptase and integrase (IN)-encoding sequences, that is not found in HIV-1 pol gene and encodes a 74-residue polypeptide with homology to dUTPases. We have expressed two BIV IN versions that differ in their amino termini. The longer version, containing the 74-residue sequence, did not show any detectable 3′-end processing and strand transfer IN activities and performed only the IN-associated disintegration. Consequently, the shorter version, lacking the dUTPase-related residues, performed all three activities and is most likely similar to the viral enzyme. A comparison between BIV IN and the well-studied HIV-1 IN, with substrates that mimic the U5 LTR sequences of BIV, HIV-1 and another bovine lentivirus, Jembrana disease virus, revealed that the extra 3′-end sequence beyond the conserved "CA" is probably less important for IN activities than the sequence upstream to the "CA".

Original languageEnglish
Pages (from-to)309-321
Number of pages13
JournalVirology
Volume371
Issue number2
DOIs
StatePublished - 20 Feb 2008

Keywords

  • 3′-end processing
  • Disintegration
  • Integrase
  • LTR
  • Reverse transcriptase
  • Strand transfer
  • The lentiviruses BIV, Jembrana disease virus and HIV-1
  • dUTPase-like segment

Fingerprint

Dive into the research topics of 'Expression and characterization of the integrase of bovine immunodeficiency virus'. Together they form a unique fingerprint.

Cite this