Expression and characterization of a novel reverse transcriptase of the LTR retrotransposon Tf1

Noa Kirshenboim, Zvi Hayouka, Assaf Friedler, Amnon Hizi

Research output: Contribution to journalArticlepeer-review

Abstract

The LTR retrotransposon of Schizosacharomyces pombe, Tf1, has several distinctive properties that can be related to the unique properties of its reverse transcriptase (RT). Consequently, we expressed, purified and studied the recombinant Tf1 RT. This monomeric protein possesses all activities typical to RTs: DNA and RNA-dependent DNA polymerase as well as an inherent ribonuclease H. The DNA polymerase activity shows preference to Mn+ 2 or Mg+ 2, depending on the substrate used, whereas the ribonuclease H strongly prefers Mn+ 2. The most outstanding feature of Tf1 RT is its capacity to add non-templated nucleotides to the 3′-ends of the nascent DNA. This is mainly apparent in the presence of Mn+ 2, as is the noticeable low fidelity of DNA synthesis. In all, Tf1 RT has a marked infidelity in synthesizing DNA at template ends, a phenomenon that can explain, as discussed herein, some of the features of Tf1 replication in the host cells.

Original languageEnglish
Pages (from-to)263-276
Number of pages14
JournalVirology
Volume366
Issue number2
DOIs
StatePublished - 30 Sep 2007

Keywords

  • DNA-dependent DNA polymerase
  • Non-templated addition of dNTPs
  • RNA-dependent DNA polymerase
  • Retroelements
  • Retroviruses
  • Reverse transcriptase
  • Ribonuclease H
  • Tf1 LTR retrotransposon

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