Exploring the cell stemness and the complexity of the adipose tissue niche

Nadav Kislev, Roza Izgilov, Raizel Adler, Dafna Benayahu

Research output: Contribution to journalArticlepeer-review

Abstract

Adipose tissue is a complex organ composed of different cellular populations, including mesenchymal stem and progenitor cells, adipocytes, and immune cells such as macrophages and lymphocytes. These cellular populations alter dynamically during aging or as a response to patho-physiology such as obesity. Changes in the various inflammatory cells are associated with metabolic complications and the development of insulin resistance, indicating that immune cells crosstalk with the adipocytes. Therefore, a study of the cell populations in the adipose tissue and the extracellular matrix maintaining the tissue niche is important for the knowledge on the regulatory state of the organ. We used a combination of methods to study various parameters to identify the composition of the resident cells in the adipose tissue and evaluate their profile. We analyzed the tissue structure and cells based on histology, immune fluorescence staining, and flow cytometry of cells present in the tissue in vivo and these markers’ expression in vitro. Any shift in cells’ composition influences self-renewal of the mesenchymal progenitors, and other cells affect the functionality of adipogenesis.

Original languageEnglish
Article number1906
JournalBiomolecules
Volume11
Issue number12
DOIs
StatePublished - Dec 2021

Keywords

  • Adipose tissue
  • Extracellular matrix
  • Immune cells
  • Mesenchymal cells

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