TY - JOUR
T1 - Exploring multisite heterogeneity of human basal cell carcinoma proteome and transcriptome
AU - Berl, Ariel
AU - Shir-Az, Ofir
AU - Genish, Ilai
AU - Biran, Hadas
AU - Mann, Din
AU - Singh, Amrita
AU - Wise, Julia
AU - Kravtsov, Vladimir
AU - Kidron, Debora
AU - Golberg, Alexander
AU - Vitkin, Edward
AU - Yakhini, Zohar
AU - Shalom, Avshalom
N1 - Publisher Copyright:
Copyright: © 2023 Berl et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2023/11
Y1 - 2023/11
N2 - Basal cell carcinoma (BCC) is the most common type of skin cancer. Due to multiple, potential underlying molecular tumor aberrations, clinical treatment protocols are not well-defined. This study presents multisite molecular heterogeneity profiles of human BCC based on RNA and proteome profiling. Three areas from lesions excised from 9 patients were analyzed. The focus was gene expression profiles based on proteome and RNA measurements of intra-tumor heterogeneity from the same patient and inter-tumor heterogeneity in nodular, infiltrative, and superficial BCC tumor subtypes from different patients. We observed significant overlap in intra- and inter-tumor variability of proteome and RNA expression profiles, showing significant multisite heterogeneity of protein expression in the BCC tumors. Inter-subtype analysis has also identified unique proteins for each BCC subtype. This profiling leads to a deeper understanding of BCC molecular heterogeneity and potentially contributes to developing new sampling tools for personalized diagnostics therapeutic approaches to BCC.
AB - Basal cell carcinoma (BCC) is the most common type of skin cancer. Due to multiple, potential underlying molecular tumor aberrations, clinical treatment protocols are not well-defined. This study presents multisite molecular heterogeneity profiles of human BCC based on RNA and proteome profiling. Three areas from lesions excised from 9 patients were analyzed. The focus was gene expression profiles based on proteome and RNA measurements of intra-tumor heterogeneity from the same patient and inter-tumor heterogeneity in nodular, infiltrative, and superficial BCC tumor subtypes from different patients. We observed significant overlap in intra- and inter-tumor variability of proteome and RNA expression profiles, showing significant multisite heterogeneity of protein expression in the BCC tumors. Inter-subtype analysis has also identified unique proteins for each BCC subtype. This profiling leads to a deeper understanding of BCC molecular heterogeneity and potentially contributes to developing new sampling tools for personalized diagnostics therapeutic approaches to BCC.
UR - http://www.scopus.com/inward/record.url?scp=85176433597&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0293744
DO - 10.1371/journal.pone.0293744
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C2 - 37948379
AN - SCOPUS:85176433597
SN - 1932-6203
VL - 18
JO - PLoS ONE
JF - PLoS ONE
IS - 11 November
M1 - e0293744
ER -
