Experimentally tested computer modeling of stress fractures in rats

Michal Stern-Perry, Amit Gefen*, Nogah Shabshin, Yoram Epstein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Experimentally tested computer modeling of stress fractures in rats. J Appl Physiol 110: 909 -916, 2011. First published February 17, 2011; doi:10.1152/japplphysiol.01468.2010.-The objective of this study was to develop a finite-element (FE) modeling methodology for studying the etiology of a stress fracture (SF). Several variants of three-dimensional FE models of a rat hindlimb, which differed in length or stiffness of tissues, enabling the analyses of mechanical strains and stress in the tibia, were created. We compared the occurrence of SFs in an animal model to validate locations of peak strains/stresses in the FE models. Four Sprague-Dawley male rats, age ̃7 wk, were subjected to mechanical cyclic loads of 1.2 Hz and ̃6 N, which were delivered to their hindlimb for 30 min, 3 times/wk, up to 12 wk, by using a specially designed apparatus. The results showed that 1) FE modeling predicted the maximal strains/stresses (̃220,0 με and ̃29 MPa, respectively) between the mid- and proximal thirds of the tibia; 2) in a longer shin, greater and more inhomogeneous tensile strains/stresses were evident, at the same location; 3) anatomical variants in shin length influenced the strain/ stress distributions to a greater extent with respect to changes in mechanical properties of tissues; and 4) bone stiffness was more dominant than muscle stiffness in affecting the strain/stress distributions. In the animal study, 35,000 loading cycles were associated with the formation of a SF. The location of the identified SF in the rat limb verified the FE model. We find the suggested model a valuable tool in studying various aspects of SFs.

Original languageEnglish
Pages (from-to)909-916
Number of pages8
JournalJournal of Applied Physiology
Issue number4
StatePublished - 1 Apr 2011


  • Bone
  • Finite element
  • Overuse injury
  • Rodent
  • Tibia


Dive into the research topics of 'Experimentally tested computer modeling of stress fractures in rats'. Together they form a unique fingerprint.

Cite this