Experimental definition of a clustered regularly interspaced short palindromic duplicon in escherichia coli

Moran G. Goren; Ido Yosef; Oren Auster; Udi Qimron

doi:10.1016/j.jmb.2012.06.037

Experimental definition of a clustered regularly interspaced short palindromic duplicon in escherichia coli

Moran G. Goren, Ido Yosef, Oren Auster, Udi Qimron^*

^*Corresponding author for this work

Clinical Microbiology and Immunology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

We analyzed sequences of newly inserted repeats in an Escherichia coli CRISPR (clustered regularly interspaced short palindromic repeats) array in vivo and showed that a base previously thought to belong to the repeat is actually derived from a protospacer. Based on further experimental results, we propose to use the term duplicon for a repeated sequence in a CRISPR array that serves as a template for a new duplicon. Our findings suggest the possibility of redrawing the borders between repeats, spacers, and protospacer adjacent motifs.

Original language	English
Pages (from-to)	14-16
Number of pages	3
Journal	Journal of Molecular Biology
Volume	423
Issue number	1
DOIs	https://doi.org/10.1016/j.jmb.2012.06.037
State	Published - 12 Oct 2012

Keywords

CRISPR adaptation
DNA template
bacteriophage
defense mechanism

Access to Document

10.1016/j.jmb.2012.06.037

Cite this

@article{c94d781afda74baf85bd1868cf8c85d9,

title = "Experimental definition of a clustered regularly interspaced short palindromic duplicon in escherichia coli",

abstract = "We analyzed sequences of newly inserted repeats in an Escherichia coli CRISPR (clustered regularly interspaced short palindromic repeats) array in vivo and showed that a base previously thought to belong to the repeat is actually derived from a protospacer. Based on further experimental results, we propose to use the term duplicon for a repeated sequence in a CRISPR array that serves as a template for a new duplicon. Our findings suggest the possibility of redrawing the borders between repeats, spacers, and protospacer adjacent motifs.",

keywords = "CRISPR adaptation, DNA template, bacteriophage, defense mechanism",

author = "Goren, {Moran G.} and Ido Yosef and Oren Auster and Udi Qimron",

note = "Funding Information: This work was supported by the Israel Science Foundation ( 611/10 ) and a Marie Curie International Reintegration Grant ( PIRG-GA-2009-256340 ).",

year = "2012",

month = oct,

day = "12",

doi = "10.1016/j.jmb.2012.06.037",

language = "אנגלית",

volume = "423",

pages = "14--16",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press",

number = "1",

}

TY - JOUR

T1 - Experimental definition of a clustered regularly interspaced short palindromic duplicon in escherichia coli

AU - Goren, Moran G.

AU - Yosef, Ido

AU - Auster, Oren

AU - Qimron, Udi

N1 - Funding Information: This work was supported by the Israel Science Foundation ( 611/10 ) and a Marie Curie International Reintegration Grant ( PIRG-GA-2009-256340 ).

PY - 2012/10/12

Y1 - 2012/10/12

N2 - We analyzed sequences of newly inserted repeats in an Escherichia coli CRISPR (clustered regularly interspaced short palindromic repeats) array in vivo and showed that a base previously thought to belong to the repeat is actually derived from a protospacer. Based on further experimental results, we propose to use the term duplicon for a repeated sequence in a CRISPR array that serves as a template for a new duplicon. Our findings suggest the possibility of redrawing the borders between repeats, spacers, and protospacer adjacent motifs.

AB - We analyzed sequences of newly inserted repeats in an Escherichia coli CRISPR (clustered regularly interspaced short palindromic repeats) array in vivo and showed that a base previously thought to belong to the repeat is actually derived from a protospacer. Based on further experimental results, we propose to use the term duplicon for a repeated sequence in a CRISPR array that serves as a template for a new duplicon. Our findings suggest the possibility of redrawing the borders between repeats, spacers, and protospacer adjacent motifs.

KW - CRISPR adaptation

KW - DNA template

KW - bacteriophage

KW - defense mechanism

UR - http://www.scopus.com/inward/record.url?scp=84866313681&partnerID=8YFLogxK

U2 - 10.1016/j.jmb.2012.06.037

DO - 10.1016/j.jmb.2012.06.037

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 22771574

AN - SCOPUS:84866313681

SN - 0022-2836

VL - 423

SP - 14

EP - 16

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 1

ER -

Experimental definition of a clustered regularly interspaced short palindromic duplicon in escherichia coli

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this