Experimental definition of a clustered regularly interspaced short palindromic duplicon in escherichia coli

Moran G. Goren, Ido Yosef, Oren Auster, Udi Qimron*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We analyzed sequences of newly inserted repeats in an Escherichia coli CRISPR (clustered regularly interspaced short palindromic repeats) array in vivo and showed that a base previously thought to belong to the repeat is actually derived from a protospacer. Based on further experimental results, we propose to use the term duplicon for a repeated sequence in a CRISPR array that serves as a template for a new duplicon. Our findings suggest the possibility of redrawing the borders between repeats, spacers, and protospacer adjacent motifs.

Original languageEnglish
Pages (from-to)14-16
Number of pages3
JournalJournal of Molecular Biology
Volume423
Issue number1
DOIs
StatePublished - 12 Oct 2012

Keywords

  • CRISPR adaptation
  • DNA template
  • bacteriophage
  • defense mechanism

Fingerprint

Dive into the research topics of 'Experimental definition of a clustered regularly interspaced short palindromic duplicon in escherichia coli'. Together they form a unique fingerprint.

Cite this