Experimental and computational protocols for studies of cross-seeding amyloid assemblies

Baiping Ren, Rundong Hu, Mingzhen Zhang, Yonglan Liu, Lijian Xu, Binbo Jiang, Jie Ma, Buyong Ma, Ruth Nussinov, Jie Zheng*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

10 Scopus citations

Abstract

Alzheimer’s disease (AD) and type 2 diabetes (T2D) are two common protein aggregation diseases. Compelling evidence has shown a link between AD and T2D, which may derive from interspecies cross-sequence interactions between amyloid-β peptide (Aβ), associated with AD, and human islet amyloid polypeptide (hIAPP), associated with T2D. Herein, we present experimental and computational protocols and tools to study the aggregate structures and kinetics, conformational conversion, and molecular interactions of Aβ-hIAPP mixtures. These protocols could be generally applied to other cross-seeding behaviors of amyloid peptides.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages429-447
Number of pages19
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1777
ISSN (Print)1064-3745

Funding

FundersFunder number
Center for Cancer Research
National Science FoundationCBET-1510099, DMR-1607475
National Institutes of HealthHHSN261200800001E
National Cancer InstituteZIABC010442
Alzheimer's Association2015-NIRG-341372
National Natural Science Foundation of ChinaNSFC-21528601

    Keywords

    • Alzheimer disease
    • Amyloid peptides
    • Cross-seeding
    • Diabetes
    • hIAPP

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