Experimental and computational protocols for studies of cross-seeding amyloid assemblies

Baiping Ren; Rundong Hu; Mingzhen Zhang; Yonglan Liu; Lijian Xu; Binbo Jiang; Jie Ma; Buyong Ma; Ruth Nussinov; Jie Zheng

doi:10.1007/978-1-4939-7811-3_27

Experimental and computational protocols for studies of cross-seeding amyloid assemblies

Baiping Ren, Rundong Hu, Mingzhen Zhang, Yonglan Liu, Lijian Xu, Binbo Jiang, Jie Ma, Buyong Ma, Ruth Nussinov, Jie Zheng^*

^*Corresponding author for this work

Human Molecular Genetics Biochemistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

14 Scopus citations

Abstract

Alzheimer’s disease (AD) and type 2 diabetes (T2D) are two common protein aggregation diseases. Compelling evidence has shown a link between AD and T2D, which may derive from interspecies cross-sequence interactions between amyloid-β peptide (Aβ), associated with AD, and human islet amyloid polypeptide (hIAPP), associated with T2D. Herein, we present experimental and computational protocols and tools to study the aggregate structures and kinetics, conformational conversion, and molecular interactions of Aβ-hIAPP mixtures. These protocols could be generally applied to other cross-seeding behaviors of amyloid peptides.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	429-447
Number of pages	19
DOIs	https://doi.org/10.1007/978-1-4939-7811-3_27
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1777
ISSN (Print)	1064-3745

Funding

Funders	Funder number
Center for Cancer Research
National Science Foundation	CBET-1510099, DMR-1607475
National Institutes of Health	HHSN261200800001E
National Cancer Institute	ZIABC010442
Alzheimer's Association	2015-NIRG-341372
National Natural Science Foundation of China	NSFC-21528601

Keywords

Alzheimer disease
Amyloid peptides
Aβ
Cross-seeding
Diabetes
hIAPP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-7811-3_27

Cite this

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Experimental and computational protocols for studies of cross-seeding amyloid assemblies

Abstract

Publication series

Funding

Keywords

UN SDGs

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