@article{3f8ce64feec3408ea9b6c0501ad2150d,
title = "Expansion of the global RNA virome reveals diverse clades of bacteriophages",
abstract = "High-throughput RNA sequencing offers broad opportunities to explore the Earth RNA virome. Mining 5,150 diverse metatranscriptomes uncovered >2.5 million RNA virus contigs. Analysis of >330,000 RNA-dependent RNA polymerases (RdRPs) shows that this expansion corresponds to a 5-fold increase of the known RNA virus diversity. Gene content analysis revealed multiple protein domains previously not found in RNA viruses and implicated in virus-host interactions. Extended RdRP phylogeny supports the monophyly of the five established phyla and reveals two putative additional bacteriophage phyla and numerous putative additional classes and orders. The dramatically expanded phylum Lenarviricota, consisting of bacterial and related eukaryotic viruses, now accounts for a third of the RNA virome. Identification of CRISPR spacer matches and bacteriolytic proteins suggests that subsets of picobirnaviruses and partitiviruses, previously associated with eukaryotes, infect prokaryotic hosts.",
keywords = "Bactriophage, Functional protein annotation, Metatranscriptomics, RNA Virus, RNA dependent RNA polymerase, Viral Ecology, Virus, Virus - Host prediction, viral phylogeny",
author = "{RNA Virus Discovery Consortium} and Uri Neri and Wolf, {Yuri I.} and Simon Roux and Camargo, {Antonio Pedro} and Benjamin Lee and Darius Kazlauskas and Chen, {I. Min} and Natalia Ivanova and {Zeigler Allen}, Lisa and David Paez-Espino and Bryant, {Donald A.} and Devaki Bhaya and Narrowe, {Adrienne B.} and Probst, {Alexander J.} and Alexander Sczyrba and Annegret Kohler and Armand S{\'e}guin and Ashley Shade and Campbell, {Barbara J.} and Lindahl, {Bj{\"o}rn D.} and Reese, {Brandi Kiel} and Roque, {Breanna M.} and Chris DeRito and Colin Averill and Daniel Cullen and Beck, {David A.C.} and Walsh, {David A.} and Ward, {David M.} and Dongying Wu and Emiley Eloe-Fadrosh and Brodie, {Eoin L.} and Young, {Erica B.} and Lilleskov, {Erik A.} and Castillo, {Federico J.} and Martin, {Francis M.} and LeCleir, {Gary R.} and Attwood, {Graeme T.} and Hinsby Cadillo-Quiroz and Simon, {Holly M.} and Ian Hewson and Grigoriev, {Igor V.} and Tiedje, {James M.} and Jansson, {Janet K.} and Janey Lee and VanderGheynst, {Jean S.} and Jeff Dangl and Bowman, {Jeff S.} and Blanchard, {Jeffrey L.} and Bowen, {Jennifer L.} and Uri Gophna",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
month = oct,
day = "13",
doi = "10.1016/j.cell.2022.08.023",
language = "אנגלית",
volume = "185",
pages = "4023--4037.e18",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier",
number = "21",
}