TY - JOUR
T1 - Expansion and redifferentiation of adult human pancreatic islet cells
AU - Ouziel-Yahalom, Limor
AU - Zalzman, Michal
AU - Anker-Kitai, Leeat
AU - Knoller, Sarah
AU - Bar, Yael
AU - Glandt, Mariela
AU - Herold, Kevan
AU - Efrat, Shimon
N1 - Funding Information:
We thank D. Steiner and C. White for antibodies, and M. Korostishevsky for assistance with statistical analysis. The Human Islet Isolation Center at Columbia University is funded by Grants U42RR016629 and P30 DK063608 from the National Institutes of Health. This work was performed in partial fulfillment of the requirements for a Ph.D. degree of Limor Ouziel-Yahalom.
PY - 2006/3/10
Y1 - 2006/3/10
N2 - β-cell replacement represents the ultimate cure for type 1 diabetes, however it is limited by availability of organ donors. Adult human islets are difficult to propagate in culture, and efforts to expand them result in dedifferentiation. Here we describe conditions for expansion of adult human islet cells, as well as a way for their redifferentiation. Most cells in islets isolated from human pancreata were induced to replicate within the first week of culture in expansion medium. Cells were propagated for 16 population doublings, without a change in replication rate or noticeable cell mortality, representing an expansion of over 65,000-fold. Replication was accompanied by a decrease in expression of key β-cell genes. Shift of the cells to differentiation medium containing betacellulin resulted in redifferentiation, as manifested by restoration of β-cell gene expression and insulin content. These methods may allow transplantation of functional islet cells from single donors into multiple recipients.
AB - β-cell replacement represents the ultimate cure for type 1 diabetes, however it is limited by availability of organ donors. Adult human islets are difficult to propagate in culture, and efforts to expand them result in dedifferentiation. Here we describe conditions for expansion of adult human islet cells, as well as a way for their redifferentiation. Most cells in islets isolated from human pancreata were induced to replicate within the first week of culture in expansion medium. Cells were propagated for 16 population doublings, without a change in replication rate or noticeable cell mortality, representing an expansion of over 65,000-fold. Replication was accompanied by a decrease in expression of key β-cell genes. Shift of the cells to differentiation medium containing betacellulin resulted in redifferentiation, as manifested by restoration of β-cell gene expression and insulin content. These methods may allow transplantation of functional islet cells from single donors into multiple recipients.
KW - Betacellulin
KW - Cell replication
KW - Dedifferentiation
KW - Insulin production
KW - Pancreatic beta cells
UR - http://www.scopus.com/inward/record.url?scp=31444455909&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.12.187
DO - 10.1016/j.bbrc.2005.12.187
M3 - מאמר
AN - SCOPUS:31444455909
VL - 341
SP - 291
EP - 298
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -