Evolutionarily conserved human targets of adenosine to inosine RNA editing

Erez Y. Levanon*, Martina Hallegger, Yaron Kinar, Ronen Shemesh, Kristina Djinovic-Carugo, Gideon Rechavi, Michael F. Jantsch, Eli Eisenberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A-to-I RNA editing by ADARs is a post-transcriptional mechanism for expanding the proteomic repertoire. Genetic recoding by editing was so far observed for only a few mammalian RNAs that are predominantly expressed in nervous tissues. However, as these editing targets fail to explain the broad and severe phenotypes of ADAR1 knockout mice, additional targets for editing by ADARs were always expected. Using comparative genomics and expressed sequence analysis, we identified and experimentally verified four additional candidate human substrates for ADAR-mediated editing: FLNA, BLCAP, CYFIP2 and IGFBP7. Additionally, editing of three of these substrates was verified in the mouse while two of them were validated in chicken. Interestingly, none of these substrates encodes a receptor protein but two of them are strongly expressed in the CNS and seem important for proper nervous system function. The editing pattern observed suggests that some of the affected proteins might have altered physiological properties leaving the possibility that they can be related to the phenotypes of ADAR1 knockout mice.

Original languageEnglish
Pages (from-to)1162-1168
Number of pages7
JournalNucleic Acids Research
Volume33
Issue number4
DOIs
StatePublished - 2005

Funding

FundersFunder number
Compugen Ltd.
Austrian Science FundSFB1706

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