Evidence that 2-allyl-2-isopropylacetamide and phenobarbital induce the same cytochrome P-450 in cultured chick embryo hepatocytes

Lea Oron*, Shoshana Bar-Nun

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The induction of cytochrome P-450 in cultured chick embryo hepatocytes was studied using two structurally unrelated compounds, 2-allyl-2-isopropylacetamide and phenobarbital. Pulse-labeling of these cells showed enhanced denovo synthesis of cytochrome P-450. The cytochrome induced by 2-allyl-2-isopropylacetamide, as well as the one induced by phenobarbital, reacted immunologically with antibodies raised against the major hepatic phenobarbital-induced isozyme. Additional form of cytochrome P-450 is induced exclusively by phenobarbital. These results clearly demonstrate that these two drugs induce at least one form of cytochrome P-450 in common.

Original languageEnglish
Pages (from-to)1096-1102
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume125
Issue number3
DOIs
StatePublished - 28 Dec 1984

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