@article{9ef36524961642139acb5820872bd7d4,
title = "Evidence for the existence of RNA of Ca2+-channel α2/δ subunit in Xenopus oocytes",
abstract = "Ba2+-currents (IBa) through voltage-dependent Ca2+-channels were studied in Xenopus oocytes injected with RNA from several excitable tissues, using the two-electrode voltage-clamp technique. Previous studies have shown that the expression of cardiac Ca2+-channels can be suppressed by an hybrid-arrest procedure that includes co-injection of the tissue-derived RNA with an 'antisense' oligonucleotide complementary to a part of RNA coding for the Ca2+-channel α1 subunit. In this study, this method was used to investigate the role of the α2/δ subunit. Co-injection of RNA extracted from either rabbit heart, rat brain or rat skeletal muscle (SkM) with 'antisense' oligonucleotides complementary to the α2/δ subunit RNA did not substantially affect the expression of IBa in the oocytes. Using the Northern blot hybridization method, it was shown that native oocytes contain large amounts of α2/δ subunit RNA of Ca2+-channel. It is proposed that te oligonucleotide treatment fails to eliminate the α2/δ RNA because of the vast excess of endogenous α2/δ RNA. These results impose a limit on the use of the hybrid-arrest method.",
keywords = "(X. laevis), Calcium channel, Hybrid-arrest method, Oocyte, RNA",
author = "Dafna Singer-Lahat and Ilana Lotan and Kiyoshi Itagaki and Arnold Schwartz and Nathan Dascal",
note = "Funding Information: We are grateful to Dr. Terry Snotch for supplying the oligonucleotides a2-1 and a2-2. This study was supported in part by the Muscular Dystrophy Assucia-tion (N.D., I.L.) and National Institutes of health grants POI-HL22619-14 (I-C), T32-HL07382-14 and R37-HL43231 (A.S.). We thank Bayer AG for supplying (-) Bay K 8644.",
year = "1992",
month = oct,
day = "6",
doi = "10.1016/0167-4889(92)90097-U",
language = "אנגלית",
volume = "1137",
pages = "39--44",
journal = "BBA - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier B.V.",
number = "1",
}