TY - JOUR
T1 - Evidence for MU-, delta-, and kappa-opioid receptors in a human neuroblastoma cell line
AU - Baumhaker, Y.
AU - Wollman, Y.
AU - Goldstein, M. N.
AU - Sarne, Y.
N1 - Funding Information:
This study was supported by the Anti-Drug Authority of Israel, and by the Ben-Gurion Fund of the Histadrut, Israel. The study was carried out as a partial requirement for the Ph.D. thesis of Y.B.
PY - 1993
Y1 - 1993
N2 - Human neuroblastoma cells were tested for the presence of opioid receptors. [3H]Diprenorphine binds to NMB cell membranes with a KD value of 0.46±0.13nM and Bmax of 534±22 fmol./mg protein. The presence of μ, δ, and k{cyrillic} opioid receptors was tested by displacing [3H]diprenorphine specific binding by the selective agonists DAMGO, DPDPE, and U50, 488H, respectively. Using this procedure, the data suggest that the NMB neuroblastoma cells express the three opioid receptor types with the abundance of δ receptors (about 60%) and minor, yet substantial populations of μ and k{cyrillic} receptors (about 20% each).
AB - Human neuroblastoma cells were tested for the presence of opioid receptors. [3H]Diprenorphine binds to NMB cell membranes with a KD value of 0.46±0.13nM and Bmax of 534±22 fmol./mg protein. The presence of μ, δ, and k{cyrillic} opioid receptors was tested by displacing [3H]diprenorphine specific binding by the selective agonists DAMGO, DPDPE, and U50, 488H, respectively. Using this procedure, the data suggest that the NMB neuroblastoma cells express the three opioid receptor types with the abundance of δ receptors (about 60%) and minor, yet substantial populations of μ and k{cyrillic} receptors (about 20% each).
UR - http://www.scopus.com/inward/record.url?scp=0027518770&partnerID=8YFLogxK
U2 - 10.1016/0024-3205(93)90061-7
DO - 10.1016/0024-3205(93)90061-7
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AN - SCOPUS:0027518770
SN - 0024-3205
VL - 52
SP - PL205-PL210
JO - Life Sciences
JF - Life Sciences
IS - 19
ER -
