Evidence for different purinergic receptors for ATP and ADP in rabbit kidney and heart

Michal Schwartzman; Rachel Pinkas; Amiram Raz

doi:10.1016/0014-2999(81)90527-6

Evidence for different purinergic receptors for ATP and ADP in rabbit kidney and heart

Michal Schwartzman, Rachel Pinkas, Amiram Raz^*

^*Corresponding author for this work

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

ATP and ADP stimulated the release of specific prostaglandin products from the perfused rabbit kidney and heart. The two nucleotides produced the same qualitative profile of prostaglandin products. In kidney, prostaglandin E₂ was the major product, whereas in heart 6-keto prostaglandin F_1α and prostaglandin E₂ predominated. ATP was slightly more potent agonist than ADP. ATP administered into the perfused heart or kidney was rapidly hydrolyzed to ADP and AMP. The prostaglandin E₂ generating activity of ATP was increased 6-10 fold when ATP was given together with AMP-PCP or AMP-PNP which competitively inhibit the activity of vascular ATPase. Thus, the rapid hydrolysis of ATP reduces its agonistic activity for prostaglandin release. ATP and ADP administered together at maximal stimulating doses produced an additive response for prostaglandin E₂ release. These results and the results of tachyphylaxis experiments indicate that ATP and ADP interact independently with different types of purinergic receptors.

Original language	English
Pages (from-to)	167-173
Number of pages	7
Journal	European Journal of Pharmacology
Volume	74
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-2999(81)90527-6
State	Published - 11 Sep 1981

Keywords

ADP
ATP
Arachidoni
Lipolysis
Prostaglandins
Purinergic

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10.1016/0014-2999(81)90527-6

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title = "Evidence for different purinergic receptors for ATP and ADP in rabbit kidney and heart",

abstract = "ATP and ADP stimulated the release of specific prostaglandin products from the perfused rabbit kidney and heart. The two nucleotides produced the same qualitative profile of prostaglandin products. In kidney, prostaglandin E2 was the major product, whereas in heart 6-keto prostaglandin F1α and prostaglandin E2 predominated. ATP was slightly more potent agonist than ADP. ATP administered into the perfused heart or kidney was rapidly hydrolyzed to ADP and AMP. The prostaglandin E2 generating activity of ATP was increased 6-10 fold when ATP was given together with AMP-PCP or AMP-PNP which competitively inhibit the activity of vascular ATPase. Thus, the rapid hydrolysis of ATP reduces its agonistic activity for prostaglandin release. ATP and ADP administered together at maximal stimulating doses produced an additive response for prostaglandin E2 release. These results and the results of tachyphylaxis experiments indicate that ATP and ADP interact independently with different types of purinergic receptors.",

keywords = "ADP, ATP, Arachidoni, Lipolysis, Prostaglandins, Purinergic",

author = "Michal Schwartzman and Rachel Pinkas and Amiram Raz",

note = "Funding Information: The skillful technical assistance of Miss Dvora Edelman and Mr. Eliezer Liberman is gratefully acknowledged. This work was supported by a grant from the U.S.-Israel Binational Science Foundation (BSF), Jerusalem, Israel.",

year = "1981",

month = sep,

day = "11",

doi = "10.1016/0014-2999(81)90527-6",

language = "אנגלית",

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T1 - Evidence for different purinergic receptors for ATP and ADP in rabbit kidney and heart

AU - Schwartzman, Michal

AU - Pinkas, Rachel

AU - Raz, Amiram

N1 - Funding Information: The skillful technical assistance of Miss Dvora Edelman and Mr. Eliezer Liberman is gratefully acknowledged. This work was supported by a grant from the U.S.-Israel Binational Science Foundation (BSF), Jerusalem, Israel.

PY - 1981/9/11

Y1 - 1981/9/11

N2 - ATP and ADP stimulated the release of specific prostaglandin products from the perfused rabbit kidney and heart. The two nucleotides produced the same qualitative profile of prostaglandin products. In kidney, prostaglandin E2 was the major product, whereas in heart 6-keto prostaglandin F1α and prostaglandin E2 predominated. ATP was slightly more potent agonist than ADP. ATP administered into the perfused heart or kidney was rapidly hydrolyzed to ADP and AMP. The prostaglandin E2 generating activity of ATP was increased 6-10 fold when ATP was given together with AMP-PCP or AMP-PNP which competitively inhibit the activity of vascular ATPase. Thus, the rapid hydrolysis of ATP reduces its agonistic activity for prostaglandin release. ATP and ADP administered together at maximal stimulating doses produced an additive response for prostaglandin E2 release. These results and the results of tachyphylaxis experiments indicate that ATP and ADP interact independently with different types of purinergic receptors.

AB - ATP and ADP stimulated the release of specific prostaglandin products from the perfused rabbit kidney and heart. The two nucleotides produced the same qualitative profile of prostaglandin products. In kidney, prostaglandin E2 was the major product, whereas in heart 6-keto prostaglandin F1α and prostaglandin E2 predominated. ATP was slightly more potent agonist than ADP. ATP administered into the perfused heart or kidney was rapidly hydrolyzed to ADP and AMP. The prostaglandin E2 generating activity of ATP was increased 6-10 fold when ATP was given together with AMP-PCP or AMP-PNP which competitively inhibit the activity of vascular ATPase. Thus, the rapid hydrolysis of ATP reduces its agonistic activity for prostaglandin release. ATP and ADP administered together at maximal stimulating doses produced an additive response for prostaglandin E2 release. These results and the results of tachyphylaxis experiments indicate that ATP and ADP interact independently with different types of purinergic receptors.

KW - ADP

KW - ATP

KW - Arachidoni

KW - Lipolysis

KW - Prostaglandins

KW - Purinergic

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VL - 74

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EP - 173

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 2-3

ER -

Evidence for different purinergic receptors for ATP and ADP in rabbit kidney and heart

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Keywords

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