Evidence for different purinergic receptors for ATP and ADP in rabbit kidney and heart

Michal Schwartzman, Rachel Pinkas, Amiram Raz

Research output: Contribution to journalArticlepeer-review

Abstract

ATP and ADP stimulated the release of specific prostaglandin products from the perfused rabbit kidney and heart. The two nucleotides produced the same qualitative profile of prostaglandin products. In kidney, prostaglandin E2 was the major product, whereas in heart 6-keto prostaglandin F and prostaglandin E2 predominated. ATP was slightly more potent agonist than ADP. ATP administered into the perfused heart or kidney was rapidly hydrolyzed to ADP and AMP. The prostaglandin E2 generating activity of ATP was increased 6-10 fold when ATP was given together with AMP-PCP or AMP-PNP which competitively inhibit the activity of vascular ATPase. Thus, the rapid hydrolysis of ATP reduces its agonistic activity for prostaglandin release. ATP and ADP administered together at maximal stimulating doses produced an additive response for prostaglandin E2 release. These results and the results of tachyphylaxis experiments indicate that ATP and ADP interact independently with different types of purinergic receptors.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalEuropean Journal of Pharmacology
Volume74
Issue number2-3
DOIs
StatePublished - 11 Sep 1981

Keywords

  • ADP
  • ATP
  • Arachidoni
  • Lipolysis
  • Prostaglandins
  • Purinergic

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