Abstract
The number of cells in the S-phase fraction of the cell cycle reflects proliferative activity. Using flow cytometry histograms and the Phoenix M+ cell cycle program, the percent of cells in the S-phase fraction was measured in single cell suspensions prepared from testes of hamsters of different ages. A cyclical pattern with a period of 9 days, superimposed on another rhythm with a 38 day period was observed (p < 0.01) during hamster maturation and it disappeared after the second spermatogenic wave, where the S phase values reached a plateau. It was concluded that maturing animals passed through a stage in which testicular biological rhythm was involved. Therefore it was concluded that it takes approximately two spermatogenic waves before the proliferation rate in the testis reached a steady state.
Original language | English |
---|---|
Pages (from-to) | 1119-1126 |
Number of pages | 8 |
Journal | Life Sciences |
Volume | 74 |
Issue number | 9 |
DOIs | |
State | Published - 16 Jan 2004 |
Keywords
- Golden hamster
- Proliferation
- S-phase
- Spermatogonia