Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in enterobacteriaceae

Liat Ashkenazi Hoffnung; Ela Burdelova; Amos Adler

doi:10.1099/jmm.0.000618

Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in enterobacteriaceae

Liat Ashkenazi Hoffnung
, Ela Burdelova
, Amos Adler^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes bla_KPC, bla_VIM, bla_NDM, bla_IMP and bla_OXA-48-carbapenemasein a collection of 96 carbapenemresistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100% sensitivity and specificity.

Original language	English
Article number	000618
Pages (from-to)	1612-1615
Number of pages	4
Journal	Journal of Medical Microbiology
Volume	66
Issue number	11
DOIs	https://doi.org/10.1099/jmm.0.000618
State	Published - Nov 2017

Keywords

Carbapenem-resistant enterobacteriaceae
Carbapenemase
Infection control
Molecular diagnostics

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10.1099/jmm.0.000618

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title = "Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in enterobacteriaceae",

abstract = "The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenemresistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100\% sensitivity and specificity.",

keywords = "Carbapenem-resistant enterobacteriaceae, Carbapenemase, Infection control, Molecular diagnostics",

author = "\{Ashkenazi Hoffnung\}, Liat and Ela Burdelova and Amos Adler",

note = "Publisher Copyright: {\textcopyright} 2017 The Authors.",

year = "2017",

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doi = "10.1099/jmm.0.000618",

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AU - Adler, Amos

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N2 - The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenemresistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100% sensitivity and specificity.

AB - The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenemresistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100% sensitivity and specificity.

KW - Carbapenem-resistant enterobacteriaceae

KW - Carbapenemase

KW - Infection control

KW - Molecular diagnostics

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ER -

Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in enterobacteriaceae

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