TY - JOUR
T1 - Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in enterobacteriaceae
AU - Ashkenazi Hoffnung, Liat
AU - Burdelova, Ela
AU - Adler, Amos
N1 - Publisher Copyright:
© 2017 The Authors.
PY - 2017/11
Y1 - 2017/11
N2 - The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenemresistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100% sensitivity and specificity.
AB - The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenemresistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100% sensitivity and specificity.
KW - Carbapenem-resistant enterobacteriaceae
KW - Carbapenemase
KW - Infection control
KW - Molecular diagnostics
UR - http://www.scopus.com/inward/record.url?scp=85033399225&partnerID=8YFLogxK
U2 - 10.1099/jmm.0.000618
DO - 10.1099/jmm.0.000618
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C2 - 29034864
AN - SCOPUS:85033399225
SN - 0022-2615
VL - 66
SP - 1612
EP - 1615
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
IS - 11
M1 - 000618
ER -