Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in enterobacteriaceae

Liat Ashkenazi Hoffnung, Ela Burdelova, Amos Adler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenemresistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100% sensitivity and specificity.

Original languageEnglish
Article number000618
Pages (from-to)1612-1615
Number of pages4
JournalJournal of Medical Microbiology
Volume66
Issue number11
DOIs
StatePublished - Nov 2017

Keywords

  • Carbapenem-resistant enterobacteriaceae
  • Carbapenemase
  • Infection control
  • Molecular diagnostics

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