Evaluation of putative molecular biomarkers in abdominal and retroperitoneal leiomyosarcomas

B. Shpitz, V. Tiomkin, Y. Bomstein, M. Gralkin, H. Buklan, J. Bernheim, E. Klein

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: Leiomyosarcomas (LMS) are diverse tumours with different biological behaviour. To evaluate the biological nature of intraabdominal and retroperitoneal leiomyosarcomas we retrospectively examined the immunoreactivity of p53, bcl-2 and proliferative activity expressed as Ki-67-labelling index in 43 tumours. Methods: Immunohistochemical staining was performed using a peroxidase-streptavidin method on paraffin-embedded sections using specific anti-p53, anti-bcl-2 and anti Ki-67 monoclonal antibodies. Results: Of 43 tumours, seven were located in the stomach, 11 in the small or large bowel, 12 in the uterus, 11 in the retroperitoneum and two cases in the urinary bladder. Five-year disease-free survival was 46.5%. Twenty-three patients (53.4%) died of the disease. Positive immunoreactivity for p53 and bcl-2 was found in 18 (41.9%) and 26 patients (60.5%), respectively. Positive Ki-67 staining was observed in eight patients (18.6%). Proliferative indices were higher in LMS with high mitotic activity (P=0.004) and high grAde (P=0.009). All Ki-67 positive LMS were intermediate or high-grade tumours. Ki-67-labelling index showed inverse relationship to bcl-2 expression. A trend towards higher survival and expression of bcl-2, p53 or Ki-67 was found. Conclusions: Our results demonstrate that p53 and bcl-2 are expressed in a substantial number of intraabdominal and retroperitoneal leiomyosarcomas. In our study, the expression of these biomarkers did not predict patient outcome. Higher Ki-67 labelling indices were found in more biologically aggressive leiomyosarcomas.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalEuropean Journal of Surgical Oncology
Volume27
Issue number2
DOIs
StatePublished - 2001

Keywords

  • Leiomyosarcoma proliferation
  • Molecular biomarkers

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