Evaluation of ovarian reserve in young females with non-iatrogenic ovarian insufficiency to establish criteria for ovarian tissue cryopreservation

Michal Zajicek, Alexander Volodarsky-Perel, Daniel Shai, Daniela Dick-Necula, Hila Raanani, Noah Gruber, Gideon Karplus, Eran Kassif, Boaz Weisz, Dror Meirow*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Research question: Can ovarian reserve parameters predict the outcome of ovarian tissue cryopreservation (OTCP) in patients ≤18 years with non-iatrogenic premature ovarian insufficiency (POI)? Design: Retrospective cohort analysis carried out in a single tertiary hospital between August 2010 and January 2020. Thirty-seven patients ≤18 years with non-iatrogenic POI (27 with Turner syndrome, six with POI of unknown aetiology, three with galactosemia and one with blepharophimosis, ptosis, epicanthus inversus syndrome) were included. Three parameters were used to evaluate ovarian reserve: anti-Müllerian hormone (AMH), follicle-stimulating hormone (FSH) and transabdominal antral follicle count. Fertility preservation (most commonly OTCP) was offered if ovarian reserve was diminished and one or more parameters was positive. Follicles were counted in ovarian samples obtained at the time of OTCP. Results: Ovarian reserve was diminished in 34 patients and 19 of them had one or more positive parameter. Fourteen (11 aged ≥12 years and 3 aged <12) underwent OTCP, one (14 years old) underwent ovarian stimulation and oocyte cryopreservation and four declined fertility preservation. Follicles were detected in 11 of 14 patients who underwent OTCP with one or more positive parameters (79%), and in all those (100%) who had two or three positive parameters. The median number of follicles was 27 (range 5–64) and 48 (range 21–75) in patients ≥12 years and those <12 years, respectively. Conclusion: This study shows that if OTCP is performed in patients with one or more positive parameters of ovarian activity, a 79% positive predictive value is achieved for the detection of follicles. The incorporation of this criterion for OTCP will minimize the risk of harvesting ovarian tissue with a low number of follicles.

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalReproductive BioMedicine Online
Issue number1
StatePublished - Jul 2023


  • Adolescence
  • Fertility preservation
  • Ovarian tissue cryopreservation
  • Premature ovarian insufficiency
  • Turner syndrome


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