Evaluation of Biotrin, Serion and Euroimmun commercial assays for the detection of parvovirus B19-specific IgM and IgG antibodies

Moran Szwarcwort-Cohen, Marina Wax, Ora Halutz, Ella Mendelson, Orna Mor*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Diagnosis of parvovirus B19 (B19) infection in small-medium size clinical laboratories is most often done by nonautomated enzyme immunoassays (EIAs). Using 195 specimens we compared the analytical performance of Biotrin (Dublin, Ireland), Euroimmun (Lubeck, Germany), and Serion (Würzburg, Germany) EIAs. Sensitivity, specificity, and concordance to Biotrin assay were calculated. Overall complete agreement in the IgG and IgM results was 88.7% (173/195) and 75.9% (148/195) samples, respectively. When equivocal results were considered positive, Serion and Euroimmun highly agreed (>93.8%) with Biotrin in the IgG serology. Serion had better IgM sensitivity and specificity than Euroimmun when compared to Biotrin, although more Serion IgM equivocal results needed reflex testing. Clinical interpretation by all three assays was identical in 83% of the samples. We concluded that overall the performance of these assays was similar and both Serion and Euroimmun could be a suitable replacement for the Biotrin.

Original languageEnglish
Article number115723
JournalDiagnostic Microbiology and Infectious Disease
Volume103
Issue number4
DOIs
StatePublished - Aug 2022

Keywords

  • Biotrin
  • Euroimmun
  • IgG serology
  • IgM serology
  • Parvovirus B19
  • Serion

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