Evaluation of an accelerated protocol for detection of extended-spectrum β-lactamase-producing gram-negative bacilli from positive blood cultures

Shiri Navon-Venezia, Azita Leavitt, Ronen Ben-Ami, Yuval Aharoni, Mitchell J. Schwaber, David Schwartz, Yehuda Carmeli*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We evaluated a protocol for the accelerated detection of extended-spectrum β-lactamases (ESBLs) in gram-negative bloodstream pathogens. Two hundred eighty-three blood culture bottles were subjected to direct ESBL testing by inoculating samples directly from blood culture bottles onto agar plates containing cefotaxime and ceftazidime disks, with and without clavulanate. Standard ESBL testing in accordance with the NCCLS guidelines after subculturing on agar plates was performed in parallel. Results of the direct ESBL testing were reported 2.3 days sooner and were comparable to those of the standard NCCLS method with sensitivity, specificity, and positive and negative predictive values of 100, 98, 94, and 100%, respectively.

Original languageEnglish
Pages (from-to)439-441
Number of pages3
JournalJournal of Clinical Microbiology
Volume43
Issue number1
DOIs
StatePublished - Jan 2005

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