TY - JOUR
T1 - Evaluation of an accelerated protocol for detection of extended-spectrum β-lactamase-producing gram-negative bacilli from positive blood cultures
AU - Navon-Venezia, Shiri
AU - Leavitt, Azita
AU - Ben-Ami, Ronen
AU - Aharoni, Yuval
AU - Schwaber, Mitchell J.
AU - Schwartz, David
AU - Carmeli, Yehuda
PY - 2005/1
Y1 - 2005/1
N2 - We evaluated a protocol for the accelerated detection of extended-spectrum β-lactamases (ESBLs) in gram-negative bloodstream pathogens. Two hundred eighty-three blood culture bottles were subjected to direct ESBL testing by inoculating samples directly from blood culture bottles onto agar plates containing cefotaxime and ceftazidime disks, with and without clavulanate. Standard ESBL testing in accordance with the NCCLS guidelines after subculturing on agar plates was performed in parallel. Results of the direct ESBL testing were reported 2.3 days sooner and were comparable to those of the standard NCCLS method with sensitivity, specificity, and positive and negative predictive values of 100, 98, 94, and 100%, respectively.
AB - We evaluated a protocol for the accelerated detection of extended-spectrum β-lactamases (ESBLs) in gram-negative bloodstream pathogens. Two hundred eighty-three blood culture bottles were subjected to direct ESBL testing by inoculating samples directly from blood culture bottles onto agar plates containing cefotaxime and ceftazidime disks, with and without clavulanate. Standard ESBL testing in accordance with the NCCLS guidelines after subculturing on agar plates was performed in parallel. Results of the direct ESBL testing were reported 2.3 days sooner and were comparable to those of the standard NCCLS method with sensitivity, specificity, and positive and negative predictive values of 100, 98, 94, and 100%, respectively.
UR - http://www.scopus.com/inward/record.url?scp=11844283366&partnerID=8YFLogxK
U2 - 10.1128/JCM.43.1.439-441.2005
DO - 10.1128/JCM.43.1.439-441.2005
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C2 - 15635009
AN - SCOPUS:11844283366
SN - 0095-1137
VL - 43
SP - 439
EP - 441
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 1
ER -