Eupergit C-coated membranes as solid support for a sensitive immunoassay of human albumin

Beka Solomon, Stephan Schmitt, Fidi Schwartz, Alma Levi, Gideon Fleminger

Research output: Contribution to journalArticlepeer-review

Abstract

A number of commercially available membranes preactivated by coating with oxirane-containing Eupergit C beads were used for diagnostic immunoassay applications. These membranes possess a higher capacity for protein binding than the respective unmodified membranes as measured with fluorescently labelled antibodies. Immobilization of antigens or antibodies as the first step of the assay was achieved by covalent binding of amino groups of proteins to the oxirane moieties introduced onto the membranes. The high performance of the newly developed membranes bearing covalently bound antibodies, was demonstrated by a dot enzyme-linked microalbuminuria immunoassay as compared to unmodified membranes.

Original languageEnglish
Pages (from-to)209-215
Number of pages7
JournalJournal of Immunological Methods
Volume157
Issue number1-2
DOIs
StatePublished - 4 Jan 1993

Keywords

  • Coated membrane
  • Covalent linkage
  • Immunoassay
  • Microalbuminuria

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