TY - JOUR
T1 - Estrone-3-glucuronide chemiluminescence immunoassay
T2 - An alternative method for monitoring induction of ovulation with human menopausal gonadotropin in an in vitro fertilization program
AU - Lessing, J. B.
AU - Peyser, M. R.
AU - Gilad, S.
AU - Amit, A.
AU - Kogosowski, A.
AU - Yovel, I.
AU - Barak, Y.
AU - David, M. P.
PY - 1987
Y1 - 1987
N2 - A simple, rapid, and sensitive solid-phase immunoassay procedure for the determination of estrone-3-glucuronide (E1-3-G), which uses chemiluminescence as the end point in unextracted morning urine, is described. Thirty-one patients undergoing induction of ovulation in an in vitro fertilization (IVF) unit participated in the study. From day 3 of the menstrual cycle until the day of hCG administration, morning blood samples and morning urine specimens were collected for the determination of serum 17β-estradiol (E2) and urine E1-3-G, respectively. A good correlation was noted between E2 measured by radioimmunoassay (RIA) and the E1-3-G measured by chemiluminescence immunoassay (CIA), from day 5 up to the day of hCG administration (0.6 < r < 0.85, P < 0.001). It is evident from this study that the CIA measurement of E1-3-G in morning urine is an accurate and rapid (2.5 hours) method and is convenient for monitoring induction of ovulation with human menopausal gonadotropins.
AB - A simple, rapid, and sensitive solid-phase immunoassay procedure for the determination of estrone-3-glucuronide (E1-3-G), which uses chemiluminescence as the end point in unextracted morning urine, is described. Thirty-one patients undergoing induction of ovulation in an in vitro fertilization (IVF) unit participated in the study. From day 3 of the menstrual cycle until the day of hCG administration, morning blood samples and morning urine specimens were collected for the determination of serum 17β-estradiol (E2) and urine E1-3-G, respectively. A good correlation was noted between E2 measured by radioimmunoassay (RIA) and the E1-3-G measured by chemiluminescence immunoassay (CIA), from day 5 up to the day of hCG administration (0.6 < r < 0.85, P < 0.001). It is evident from this study that the CIA measurement of E1-3-G in morning urine is an accurate and rapid (2.5 hours) method and is convenient for monitoring induction of ovulation with human menopausal gonadotropins.
UR - http://www.scopus.com/inward/record.url?scp=0023198318&partnerID=8YFLogxK
U2 - 10.1016/S0015-0282(16)59416-3
DO - 10.1016/S0015-0282(16)59416-3
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AN - SCOPUS:0023198318
SN - 0015-0282
VL - 48
SP - 450
EP - 453
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 3
ER -