@article{189f0967fa164dbdac70260451df7546,
title = "Establishment and validation of a model for non-luteinized human mural granulosa cell culture",
abstract = "Cell culture techniques of human mural granulosa cells (MGCs) serve as a major in vitro tool. However, the use of luteinized MGCs has major limitations due to their luteinized state. Our aim was to establish a standardized protocol for the culture of MGCs as a model for different stages of folliculogenesis. We showed that early-non-luteinized, preovulatory-non-luteinized and luteal-MGCs have distinct gene expression pattern. After 4. days of incubation of luteinized-MGCs, ovulatory genes mRNA's achieve expression levels similar to the early non-luteinized follicles. FSH stimulation for 48. h of these 4. days cultured MGCs showed ovulatory genes mRNA's expression similar to the pre-ovulatory non-luteinized follicles. These FSH-stimulated cells responded to hCG stimulation in a pattern similar to the response of pre-ovulatory follicles.This novel model may provide a standardized research tool for delineation of the molecular processes occurring during the latter stages of follicular development in the human ovary.",
keywords = "Cell culture, Follicle, Luteinization, Mural granulosa cells, Ovary",
author = "L. Ophir and Y. Yung and E. Maman and N. Rubinstein and Yerushalmi, {G. M.} and J. Haas and E. Barzilay and A. Hourvitz",
note = "Funding Information: This manuscript was supported by grants from the Ministry of Health, Israel to AH and from the Israel Science Foundation (ISF) to EM. ",
year = "2014",
month = mar,
day = "25",
doi = "10.1016/j.mce.2014.01.018",
language = "אנגלית",
volume = "384",
pages = "165--174",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1-2",
}