Establishment and validation of a model for non-luteinized human mural granulosa cell culture

L. Ophir, Y. Yung, E. Maman, N. Rubinstein, G. M. Yerushalmi, J. Haas, E. Barzilay, A. Hourvitz

Research output: Contribution to journalArticlepeer-review


Cell culture techniques of human mural granulosa cells (MGCs) serve as a major in vitro tool. However, the use of luteinized MGCs has major limitations due to their luteinized state. Our aim was to establish a standardized protocol for the culture of MGCs as a model for different stages of folliculogenesis. We showed that early-non-luteinized, preovulatory-non-luteinized and luteal-MGCs have distinct gene expression pattern. After 4. days of incubation of luteinized-MGCs, ovulatory genes mRNA's achieve expression levels similar to the early non-luteinized follicles. FSH stimulation for 48. h of these 4. days cultured MGCs showed ovulatory genes mRNA's expression similar to the pre-ovulatory non-luteinized follicles. These FSH-stimulated cells responded to hCG stimulation in a pattern similar to the response of pre-ovulatory follicles.This novel model may provide a standardized research tool for delineation of the molecular processes occurring during the latter stages of follicular development in the human ovary.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalMolecular and Cellular Endocrinology
Issue number1-2
StatePublished - 25 Mar 2014


  • Cell culture
  • Follicle
  • Luteinization
  • Mural granulosa cells
  • Ovary


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