Erythrocytes volume and refractive index measurement with a digital holographic microscope

B. Rappaz; A. Barbul; F. Charrière; J. Kühn; P. Marquet; R. Korenstein; C. Depeursinge; P. Magistretti

doi:10.1117/12.700463

Erythrocytes volume and refractive index measurement with a digital holographic microscope

B. Rappaz^*, A. Barbul, F. Charrière, J. Kühn, P. Marquet, R. Korenstein, C. Depeursinge, P. Magistretti

^*Corresponding author for this work

Physiology Pharmacology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

9 Scopus citations

Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible to accurately measure (with a precision of 0.0003) the mean refractive index and the volume of living erythrocytes. Here, application of this decoupling procedure on erythrocyte allows to measure a refractive index of 1.40 and a mean volume of about 106 μm³.

Original language	English
Title of host publication	Optical Diagnostics and Sensing VII
DOIs	https://doi.org/10.1117/12.700463
State	Published - 2007
Event	Optical Diagnostics and Sensing VII - San Jose, CA, United States Duration: 23 Jan 2007 → 24 Jan 2007

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6445
ISSN (Print)	1605-7422

Conference

Conference	Optical Diagnostics and Sensing VII
Country/Territory	United States
City	San Jose, CA
Period	23/01/07 → 24/01/07

Keywords

Cell imaging
Digital holography
Erythrocyte
Refractive index

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10.1117/12.700463

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Rappaz, B., Barbul, A., Charrière, F., Kühn, J., Marquet, P., Korenstein, R., Depeursinge, C., & Magistretti, P. (2007). Erythrocytes volume and refractive index measurement with a digital holographic microscope. In Optical Diagnostics and Sensing VII Article 644509 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6445). https://doi.org/10.1117/12.700463

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title = "Erythrocytes volume and refractive index measurement with a digital holographic microscope",

abstract = "Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible to accurately measure (with a precision of 0.0003) the mean refractive index and the volume of living erythrocytes. Here, application of this decoupling procedure on erythrocyte allows to measure a refractive index of 1.40 and a mean volume of about 106 μm3.",

keywords = "Cell imaging, Digital holography, Erythrocyte, Refractive index",

author = "B. Rappaz and A. Barbul and F. Charri{\`e}re and J. K{\"u}hn and P. Marquet and R. Korenstein and C. Depeursinge and P. Magistretti",

year = "2007",

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Rappaz, B, Barbul, A, Charrière, F, Kühn, J, Marquet, P, Korenstein, R, Depeursinge, C & Magistretti, P 2007, Erythrocytes volume and refractive index measurement with a digital holographic microscope. in Optical Diagnostics and Sensing VII., 644509, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6445, Optical Diagnostics and Sensing VII, San Jose, CA, United States, 23/01/07. https://doi.org/10.1117/12.700463

Erythrocytes volume and refractive index measurement with a digital holographic microscope. / Rappaz, B.; Barbul, A.; Charrière, F. et al.
Optical Diagnostics and Sensing VII. 2007. 644509 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6445).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Rappaz, B.

AU - Barbul, A.

AU - Charrière, F.

AU - Kühn, J.

AU - Marquet, P.

AU - Korenstein, R.

AU - Depeursinge, C.

AU - Magistretti, P.

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AB - Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible to accurately measure (with a precision of 0.0003) the mean refractive index and the volume of living erythrocytes. Here, application of this decoupling procedure on erythrocyte allows to measure a refractive index of 1.40 and a mean volume of about 106 μm3.

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KW - Refractive index

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Erythrocytes volume and refractive index measurement with a digital holographic microscope

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