Erythrocytes volume and refractive index measurement with a digital holographic microscope

B. Rappaz*, A. Barbul, F. Charrière, J. Kühn, P. Marquet, R. Korenstein, C. Depeursinge, P. Magistretti

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible to accurately measure (with a precision of 0.0003) the mean refractive index and the volume of living erythrocytes. Here, application of this decoupling procedure on erythrocyte allows to measure a refractive index of 1.40 and a mean volume of about 106 μm3.

Original languageEnglish
Title of host publicationOptical Diagnostics and Sensing VII
StatePublished - 2007
EventOptical Diagnostics and Sensing VII - San Jose, CA, United States
Duration: 23 Jan 200724 Jan 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceOptical Diagnostics and Sensing VII
Country/TerritoryUnited States
CitySan Jose, CA


  • Cell imaging
  • Digital holography
  • Erythrocyte
  • Refractive index


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