TY - JOUR
T1 - ERdj8 governs the size of autophagosomes during the formation process
AU - Yamamoto, Yo Hei
AU - Kasai, Ayano
AU - Omori, Hiroko
AU - Takino, Tomoe
AU - Sugihara, Munechika
AU - Umemoto, Tetsuo
AU - Hamasaki, Maho
AU - Hatta, Tomohisa
AU - Natsume, Tohru
AU - Morimoto, Richard I.
AU - Arai, Ritsuko
AU - Waguri, Satoshi
AU - Sato, Miyuki
AU - Sato, Ken
AU - Bar-Nun, Shoshana
AU - Yoshimori, Tamotsu
AU - Noda, Takeshi
AU - Nagata, Kazuhiro
N1 - Publisher Copyright:
© 2020 Yamamoto et al.
PY - 2020/8/3
Y1 - 2020/8/3
N2 - In macroautophagy, membrane structures called autophagosomes engulf substrates and deliver them for lysosomal degradation. Autophagosomes enwrap a variety of targets with diverse sizes, from portions of cytosol to larger organelles. However, the mechanism by which autophagosome size is controlled remains elusive. We characterized a novel ER membrane protein, ERdj8, in mammalian cells. ERdj8 localizes to a meshwork-like ER subdomain along with phosphatidylinositol synthase (PIS) and autophagy-related (Atg) proteins. ERdj8 overexpression extended the size of the autophagosome through its DnaJ and TRX domains. ERdj8 ablation resulted in a defect in engulfing larger targets. C. elegans, in which the ERdj8 orthologue dnj-8 was knocked down, could perform autophagy on smaller mitochondria derived from the paternal lineage but not the somatic mitochondria. Thus, ERdj8 may play a critical role in autophagosome formation by providing the capacity to target substrates of diverse sizes for degradation.
AB - In macroautophagy, membrane structures called autophagosomes engulf substrates and deliver them for lysosomal degradation. Autophagosomes enwrap a variety of targets with diverse sizes, from portions of cytosol to larger organelles. However, the mechanism by which autophagosome size is controlled remains elusive. We characterized a novel ER membrane protein, ERdj8, in mammalian cells. ERdj8 localizes to a meshwork-like ER subdomain along with phosphatidylinositol synthase (PIS) and autophagy-related (Atg) proteins. ERdj8 overexpression extended the size of the autophagosome through its DnaJ and TRX domains. ERdj8 ablation resulted in a defect in engulfing larger targets. C. elegans, in which the ERdj8 orthologue dnj-8 was knocked down, could perform autophagy on smaller mitochondria derived from the paternal lineage but not the somatic mitochondria. Thus, ERdj8 may play a critical role in autophagosome formation by providing the capacity to target substrates of diverse sizes for degradation.
UR - http://www.scopus.com/inward/record.url?scp=85085985341&partnerID=8YFLogxK
U2 - 10.1083/jcb.201903127
DO - 10.1083/jcb.201903127
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C2 - 32492081
AN - SCOPUS:85085985341
SN - 0021-9525
VL - 219
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 8
M1 - e201903127
ER -