Eradication of multidrug-resistant pseudomonas biofilm with pulsed electric fields

Saiqa I. Khan, Gaddi Blumrosen, Daniela Vecchio, Alexander Golberg, Michael C. Mccormack, Martin L. Yarmush, Michael R. Hamblin, William G. Austen

Research output: Contribution to journalArticlepeer-review

Abstract

Biofilm formation is a significant problem, accounting for over eighty percent of microbial infections in the body. Biofilm eradication is problematic due to increased resistance to antibiotics and antimicrobials as compared to planktonic cells. The purpose of this study was to investigate the effect of Pulsed Electric Fields (PEF) on biofilm-infected mesh. Prolene mesh was infected with bioluminescent Pseudomonas aeruginosa and treated with PEF using a concentric electrode system to derive, in a single experiment, the critical electric field strength needed to kill bacteria. The effect of the electric field strength and the number of pulses (with a fixed pulse length duration and frequency) on bacterial eradication was investigated. For all experiments, biofilm formation and disruption were confirmed with bioluminescent imaging and Scanning Electron Microscopy (SEM). Computation and statistical methods were used to analyze treatment efficiency and to compare it to existing theoretical models. In all experiments 1500V are applied through a central electrode, with pulse duration of 50μs, and pulse delivery frequency of 2Hz. We found that the critical electric field strength (Ecr) needed to eradicate 100-80% of bacteria in the treated area was 121±14V/mm when 300 pulses were applied, and 235±6.1V/mm when 150 pulses were applied. The area at which 100-80% of bacteria were eradicated was 50.5±9.9mm2 for 300 pulses, and 13.4±0.65mm2 for 150 pulses. 80% threshold eradication was not achieved with 100 pulses. The results indicate that increased efficacy of treatment is due to increased number of pulses delivered. In addition, we that showed the bacterial death rate as a function of the electrical field follows the statistical Weibull model for 150 and 300pulses. We hypothesize that in the clinical setting, combining systemic antibacterial therapy with PEF will yield a synergistic effect leading to improved eradication of mesh infections.

Original languageEnglish
Pages (from-to)643-650
Number of pages8
JournalBiotechnology and Bioengineering
Volume113
Issue number3
DOIs
StatePublished - 1 Mar 2016

Keywords

  • Biofilm
  • Eradication of multidrug resistant infections
  • Irreversible electroporation
  • Medical device disinfection
  • Pulsed electric fields
  • Treatment of mesh infection

Fingerprint

Dive into the research topics of 'Eradication of multidrug-resistant pseudomonas biofilm with pulsed electric fields'. Together they form a unique fingerprint.

Cite this