Epiretinal transplantation of human bone marrow mesenchymal stem cells rescues retinal and vision function in a rat model of retinal degeneration

Adi Tzameret; Ifat Sher; Michael Belkin; Avraham J. Treves; Amilia Meir; Arnon Nagler; Hani Levkovitch-Verbin; Ygal Rotenstreich; Arieh S. Solomon

doi:10.1016/j.scr.2015.08.007

Epiretinal transplantation of human bone marrow mesenchymal stem cells rescues retinal and vision function in a rat model of retinal degeneration

Adi Tzameret, Ifat Sher, Michael Belkin, Avraham J. Treves, Amilia Meir, Arnon Nagler, Hani Levkovitch-Verbin, Ygal Rotenstreich, Arieh S. Solomon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Vision incapacitation and blindness associated with incurable retinal degeneration affect millions of people worldwide. In this study, 0.25×10⁶ human bone marrow stem cells (hBM-MSCs) were transplanted epiretinally in the right eye of Royal College Surgeons (RCS) rats at the age of 28 days. Epiretinally transplanted cells were identified as a thin layer of cells along vitreous cavity, in close proximity to the retina or attached to the lens capsule, up to 6 weeks following transplantation. Epiretinal transplantation delayed photoreceptor degeneration and rescued retinal function up to 20 weeks following cell transplantation. Visual functions remained close to normal levels in epiretinal transplantation rats. No inflammation or any other adverse effects were observed in transplanted eyes.Our findings suggest that transplantation of hBM-MSCs as a thin epiretinal layer is effective for treatment of retinal degeneration in RCS rats, and that transplanting the cells in close proximity to the retina enhances hBM-MSC therapeutic effect compared with intravitreal injection.

Original language	English
Pages (from-to)	387-394
Number of pages	8
Journal	Stem Cell Research
Volume	15
Issue number	2
DOIs	https://doi.org/10.1016/j.scr.2015.08.007
State	Published - 1 Sep 2015

Keywords

Epiretinal transplantation
Human bone marrow mesenchymal stem cells
RCS rats
Retinal degeneration

Access to Document

10.1016/j.scr.2015.08.007

Cite this

@article{0ae6921aced546d1bfc3adc6a0c5e790,

title = "Epiretinal transplantation of human bone marrow mesenchymal stem cells rescues retinal and vision function in a rat model of retinal degeneration",

abstract = "Vision incapacitation and blindness associated with incurable retinal degeneration affect millions of people worldwide. In this study, 0.25×106 human bone marrow stem cells (hBM-MSCs) were transplanted epiretinally in the right eye of Royal College Surgeons (RCS) rats at the age of 28 days. Epiretinally transplanted cells were identified as a thin layer of cells along vitreous cavity, in close proximity to the retina or attached to the lens capsule, up to 6 weeks following transplantation. Epiretinal transplantation delayed photoreceptor degeneration and rescued retinal function up to 20 weeks following cell transplantation. Visual functions remained close to normal levels in epiretinal transplantation rats. No inflammation or any other adverse effects were observed in transplanted eyes.Our findings suggest that transplantation of hBM-MSCs as a thin epiretinal layer is effective for treatment of retinal degeneration in RCS rats, and that transplanting the cells in close proximity to the retina enhances hBM-MSC therapeutic effect compared with intravitreal injection.",

keywords = "Epiretinal transplantation, Human bone marrow mesenchymal stem cells, RCS rats, Retinal degeneration",

author = "Adi Tzameret and Ifat Sher and Michael Belkin and Treves, {Avraham J.} and Amilia Meir and Arnon Nagler and Hani Levkovitch-Verbin and Ygal Rotenstreich and Solomon, {Arieh S.}",

note = "Publisher Copyright: {\textcopyright} 2015.",

year = "2015",

month = sep,

day = "1",

doi = "10.1016/j.scr.2015.08.007",

language = "אנגלית",

volume = "15",

pages = "387--394",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Epiretinal transplantation of human bone marrow mesenchymal stem cells rescues retinal and vision function in a rat model of retinal degeneration

AU - Tzameret, Adi

AU - Sher, Ifat

AU - Belkin, Michael

AU - Treves, Avraham J.

AU - Meir, Amilia

AU - Nagler, Arnon

AU - Levkovitch-Verbin, Hani

AU - Rotenstreich, Ygal

AU - Solomon, Arieh S.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Vision incapacitation and blindness associated with incurable retinal degeneration affect millions of people worldwide. In this study, 0.25×106 human bone marrow stem cells (hBM-MSCs) were transplanted epiretinally in the right eye of Royal College Surgeons (RCS) rats at the age of 28 days. Epiretinally transplanted cells were identified as a thin layer of cells along vitreous cavity, in close proximity to the retina or attached to the lens capsule, up to 6 weeks following transplantation. Epiretinal transplantation delayed photoreceptor degeneration and rescued retinal function up to 20 weeks following cell transplantation. Visual functions remained close to normal levels in epiretinal transplantation rats. No inflammation or any other adverse effects were observed in transplanted eyes.Our findings suggest that transplantation of hBM-MSCs as a thin epiretinal layer is effective for treatment of retinal degeneration in RCS rats, and that transplanting the cells in close proximity to the retina enhances hBM-MSC therapeutic effect compared with intravitreal injection.

AB - Vision incapacitation and blindness associated with incurable retinal degeneration affect millions of people worldwide. In this study, 0.25×106 human bone marrow stem cells (hBM-MSCs) were transplanted epiretinally in the right eye of Royal College Surgeons (RCS) rats at the age of 28 days. Epiretinally transplanted cells were identified as a thin layer of cells along vitreous cavity, in close proximity to the retina or attached to the lens capsule, up to 6 weeks following transplantation. Epiretinal transplantation delayed photoreceptor degeneration and rescued retinal function up to 20 weeks following cell transplantation. Visual functions remained close to normal levels in epiretinal transplantation rats. No inflammation or any other adverse effects were observed in transplanted eyes.Our findings suggest that transplantation of hBM-MSCs as a thin epiretinal layer is effective for treatment of retinal degeneration in RCS rats, and that transplanting the cells in close proximity to the retina enhances hBM-MSC therapeutic effect compared with intravitreal injection.

KW - Epiretinal transplantation

KW - Human bone marrow mesenchymal stem cells

KW - RCS rats

KW - Retinal degeneration

UR - http://www.scopus.com/inward/record.url?scp=84941279369&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2015.08.007

DO - 10.1016/j.scr.2015.08.007

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:84941279369

SN - 1873-5061

VL - 15

SP - 387

EP - 394

JO - Stem Cell Research

JF - Stem Cell Research

IS - 2

ER -

Epiretinal transplantation of human bone marrow mesenchymal stem cells rescues retinal and vision function in a rat model of retinal degeneration

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this