Epiregulin is a potent Pan-ErbB ligand that preferentially activates heterodimeric receptor complexes

Maya Shelly, Ronit Pinkas-Kramarski, Bradley C. Guarino, Hadassa Waterman, Ling Mei Wang, Ljuba Lyass, Mauricio Alimandi, Angera Kuo, Sarah S. Bacus, Jacalyn H. Pierce, Glenn C. Andrews, Yosef Yarden

Research output: Contribution to journalArticlepeer-review

Abstract

The ErbB signaling network consists of four transmembrane receptor tyrosine kinases and more than a dozen ligands sharing an epidermal growth factor (EGF) motif. The multiplicity of ErbB-specific ligands is incompletely understood in terms of signal specificity because all ErbB molecules signal through partially overlapping pathways. Here we addressed the action of epiregulin, a recently isolated ligand of ErbB-1. By employing a set of factor-dependent cell lines engineered to express individual ErbBs or their combinations, we found that epiregulin is the broadest specificity EGF-like ligand so far characterized: not only does it stimulate homodimers of both ErbB-1 and ErbB-4, it also activates all possible heterodimeric ErbB complexes. Consistent with its relaxed selectivity, epiregulin binds the various receptor combinations with an affinity that is approximately 100- fold lower than the affinity of ligands with more stringent selectivity, including EGF. Nevertheless, epiregulin's action upon most receptor combinations transmits a more potent mitogenic signal than does EGF. This remarkable discrepancy between binding affinity and bioactivity is permitted by a mechanism that prevents receptor down-regulation, and results in a weak, but prolonged, state of receptor activation.

Original languageEnglish
Pages (from-to)10496-10505
Number of pages10
JournalJournal of Biological Chemistry
Volume273
Issue number17
DOIs
StatePublished - 24 Apr 1998
Externally publishedYes

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