Enzyme-free amplified detection of DNA by an autonomous ligation DNAzyme machinery

Fuan Wang, Johann Elbaz, Itamar Willner

Research output: Contribution to journalArticlepeer-review

Abstract

The Zn 2+-dependent ligation DNAzyme is implemented as a biocatalyst for the amplified detection of a target DNA by the autonomous replication of a nucleic acid reporter unit that is generated by the catalyzed ligation process. The reporter units enhance the formation of active DNAzyme units, thus leading to the isothermal autocatalytic formation of the reporter elements. The system was further developed and applied for the amplified detection of Tay-Sachs genetic disorder mutant, with a detection limit of 1.0 × 10 -11 M. Besides providing a versatile paradigm for the amplified detection of DNA, the system reveals a new, enzyme-free, isothermal, autocatalytic mechanism that introduces means for effective programmed synthesis.

Original languageEnglish
Pages (from-to)5504-5507
Number of pages4
JournalJournal of the American Chemical Society
Volume134
Issue number12
DOIs
StatePublished - 28 Mar 2012
Externally publishedYes

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