Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins

Soohong Kim, Anna Gottfried, Ron R. Lin, Thomas Dertinger, Andrew S. Kim, Sangyoon Chung, Ryan A. Colyer, Elmar Weinhold, Shimon Weiss, Yuval Ebenstein

Research output: Contribution to journalArticlepeer-review

Abstract

All smiles: Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome (see picture). The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single-molecule level.

Original languageEnglish
Pages (from-to)3578-3581
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number15
DOIs
StatePublished - 10 Apr 2012

Keywords

  • DNA
  • imaging agents
  • optical mapping
  • quantum dots
  • single-molecule studies

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