Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins

Soohong Kim, Anna Gottfried, Ron R. Lin, Thomas Dertinger, Andrew S. Kim, Sangyoon Chung, Ryan A. Colyer, Elmar Weinhold*, Shimon Weiss, Yuval Ebenstein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

All smiles: Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome (see picture). The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single-molecule level.

Original languageEnglish
Pages (from-to)3578-3581
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number15
DOIs
StatePublished - 10 Apr 2012

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM069709

    Keywords

    • DNA
    • imaging agents
    • optical mapping
    • quantum dots
    • single-molecule studies

    Fingerprint

    Dive into the research topics of 'Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins'. Together they form a unique fingerprint.

    Cite this