Enzymatic Activity Profiling Using an Ultrasensitive Array of Chemiluminescent Probes for Bacterial Classification and Characterization

Omri Shelef, Tal Kopp, Rozan Tannous, Maxence Arutkin, Moriah Jospe-Kaufman, Shlomi Reuveni*, Doron Shabat*, Micha Fridman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Identification and characterization of bacterial species in clinical and industrial settings necessitate the use of diverse, labor-intensive, and time-consuming protocols as well as the utilization of expensive and high-maintenance equipment. Furthermore, while cutting-edge identification technologies such as mass spectrometry and PCR are highly effective in identifying bacterial pathogens, they fall short in providing additional information for identifying bacteria not present in the databases upon which these methods rely. In response to these challenges, we present a robust and general approach to bacterial identification based on their unique enzymatic activity profiles. This method delivers results within 90 min, utilizing an array of highly sensitive and enzyme-selective chemiluminescent probes. Leveraging our recently developed technology of chemiluminescent luminophores, which emit light under physiological conditions, we have crafted an array of probes designed to rapidly detect various bacterial enzymatic activities. The array includes probes for detecting resistance to the important and large class of β-lactam antibiotics. The analysis of chemiluminescent fingerprints from a diverse range of prominent bacterial pathogens unveiled distinct enzymatic activity profiles for each strain. The reported universally applicable identification procedure offers a highly sensitive and expeditious means to delineate bacterial enzymatic activity fingerprints. This opens new avenues for characterizing and identifying pathogens in research, clinical, and industrial applications.

Original languageEnglish
Pages (from-to)5263-5273
Number of pages11
JournalJournal of the American Chemical Society
Issue number8
StatePublished - 28 Feb 2024


FundersFunder number
Israeli Ministry of Science, Technology & Space for the Levi Eshkol Scholarship315461
Ulysse Mizrahi
Horizon 2020 Framework Programme947731
European Research Council
Israel Academy of Sciences and Humanities
Ministry of Science and Technology, Israel0004857


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