@article{f52aaaabc11f4dcfbd4b8f12f19e5798,
title = "Enhanced transcription of the human α2A-adrenergic receptor gene by cAMP: Evidence for multiple cAMP responsive sequences in the promoter region of this gene",
abstract = "Expression of the human α2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5′-flanking sequences of the gene were placed in a promoterless vector with a chloramphenicol acetyltransferase (CAT) reporter gene, and cAMP-stimulated CAT activity was assayed in transfected JEG-3 placental carcinoma cells. Enhancer activity responsive to cAMP was located in DNA sequences both upstream and downstream from the endogenous promoter region. Within the upstream sequences there is a putative {"}core sequence{"} homologous to the eight base CRE consensus palindrome, but this region did not function independently as a CRE enhancer; additional upstream sequences were required to provide significant enhancer activity in response to cAMP. Regulation of expression of the α2A-adrenergic gene by cAMP is complex and involves multiple and likely novel DNA sequences.",
keywords = "cAMP, enhancer, gene expression, transcription, transfection, α-adrenergic receptor",
author = "Lotan Shilo and Motoyoshi Sakaue and Thomas, {John M.} and Mohan Philip and Hoffman, {Brian B.}",
note = "Funding Information: Acknowledgements--We thank J. RAMACHANDRAN for his encouragement during the course of this work and helpful advice from G. CRAn~V.~ and R. Sctm~J .~,. DR SmLO was supported by a Merck international fellowship in Clinical Pharmacology. The work was supported by NIH grant AG09597 and the VA.",
year = "1994",
month = jan,
doi = "10.1016/0898-6568(94)90062-0",
language = "אנגלית",
volume = "6",
pages = "73--82",
journal = "Cellular Signalling",
issn = "0898-6568",
publisher = "Elsevier Inc.",
number = "1",
}
