Enhanced transcription of the human α2A-adrenergic receptor gene by cAMP: Evidence for multiple cAMP responsive sequences in the promoter region of this gene

Lotan Shilo; Motoyoshi Sakaue; John M. Thomas; Mohan Philip; Brian B. Hoffman

doi:10.1016/0898-6568(94)90062-0

Enhanced transcription of the human α_2A-adrenergic receptor gene by cAMP: Evidence for multiple cAMP responsive sequences in the promoter region of this gene

Lotan Shilo, Motoyoshi Sakaue, John M. Thomas, Mohan Philip, Brian B. Hoffman

Research output: Contribution to journal › Article › peer-review

Abstract

Expression of the human α_2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5′-flanking sequences of the gene were placed in a promoterless vector with a chloramphenicol acetyltransferase (CAT) reporter gene, and cAMP-stimulated CAT activity was assayed in transfected JEG-3 placental carcinoma cells. Enhancer activity responsive to cAMP was located in DNA sequences both upstream and downstream from the endogenous promoter region. Within the upstream sequences there is a putative "core sequence" homologous to the eight base CRE consensus palindrome, but this region did not function independently as a CRE enhancer; additional upstream sequences were required to provide significant enhancer activity in response to cAMP. Regulation of expression of the α_2A-adrenergic gene by cAMP is complex and involves multiple and likely novel DNA sequences.

Original language	English
Pages (from-to)	73-82
Number of pages	10
Journal	Cellular Signalling
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/0898-6568(94)90062-0
State	Published - Jan 1994
Externally published	Yes

Keywords

cAMP
enhancer
gene expression
transcription
transfection
α-adrenergic receptor

Access to Document

10.1016/0898-6568(94)90062-0

Cite this

@article{f52aaaabc11f4dcfbd4b8f12f19e5798,

title = "Enhanced transcription of the human α2A-adrenergic receptor gene by cAMP: Evidence for multiple cAMP responsive sequences in the promoter region of this gene",

abstract = "Expression of the human α2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5′-flanking sequences of the gene were placed in a promoterless vector with a chloramphenicol acetyltransferase (CAT) reporter gene, and cAMP-stimulated CAT activity was assayed in transfected JEG-3 placental carcinoma cells. Enhancer activity responsive to cAMP was located in DNA sequences both upstream and downstream from the endogenous promoter region. Within the upstream sequences there is a putative {"}core sequence{"} homologous to the eight base CRE consensus palindrome, but this region did not function independently as a CRE enhancer; additional upstream sequences were required to provide significant enhancer activity in response to cAMP. Regulation of expression of the α2A-adrenergic gene by cAMP is complex and involves multiple and likely novel DNA sequences.",

keywords = "cAMP, enhancer, gene expression, transcription, transfection, α-adrenergic receptor",

author = "Lotan Shilo and Motoyoshi Sakaue and Thomas, {John M.} and Mohan Philip and Hoffman, {Brian B.}",

note = "Funding Information: Acknowledgements--We thank J. RAMACHANDRAN for his encouragement during the course of this work and helpful advice from G. CRAn~V.~ and R. Sctm~J .~,. DR SmLO was supported by a Merck international fellowship in Clinical Pharmacology. The work was supported by NIH grant AG09597 and the VA.",

year = "1994",

month = jan,

doi = "10.1016/0898-6568(94)90062-0",

language = "אנגלית",

volume = "6",

pages = "73--82",

journal = "Cellular Signalling",

issn = "0898-6568",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - Enhanced transcription of the human α2A-adrenergic receptor gene by cAMP

T2 - Evidence for multiple cAMP responsive sequences in the promoter region of this gene

AU - Shilo, Lotan

AU - Sakaue, Motoyoshi

AU - Thomas, John M.

AU - Philip, Mohan

AU - Hoffman, Brian B.

N1 - Funding Information: Acknowledgements--We thank J. RAMACHANDRAN for his encouragement during the course of this work and helpful advice from G. CRAn~V.~ and R. Sctm~J .~,. DR SmLO was supported by a Merck international fellowship in Clinical Pharmacology. The work was supported by NIH grant AG09597 and the VA.

PY - 1994/1

Y1 - 1994/1

N2 - Expression of the human α2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5′-flanking sequences of the gene were placed in a promoterless vector with a chloramphenicol acetyltransferase (CAT) reporter gene, and cAMP-stimulated CAT activity was assayed in transfected JEG-3 placental carcinoma cells. Enhancer activity responsive to cAMP was located in DNA sequences both upstream and downstream from the endogenous promoter region. Within the upstream sequences there is a putative "core sequence" homologous to the eight base CRE consensus palindrome, but this region did not function independently as a CRE enhancer; additional upstream sequences were required to provide significant enhancer activity in response to cAMP. Regulation of expression of the α2A-adrenergic gene by cAMP is complex and involves multiple and likely novel DNA sequences.

AB - Expression of the human α2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5′-flanking sequences of the gene were placed in a promoterless vector with a chloramphenicol acetyltransferase (CAT) reporter gene, and cAMP-stimulated CAT activity was assayed in transfected JEG-3 placental carcinoma cells. Enhancer activity responsive to cAMP was located in DNA sequences both upstream and downstream from the endogenous promoter region. Within the upstream sequences there is a putative "core sequence" homologous to the eight base CRE consensus palindrome, but this region did not function independently as a CRE enhancer; additional upstream sequences were required to provide significant enhancer activity in response to cAMP. Regulation of expression of the α2A-adrenergic gene by cAMP is complex and involves multiple and likely novel DNA sequences.

KW - cAMP

KW - enhancer

KW - gene expression

KW - transcription

KW - transfection

KW - α-adrenergic receptor

UR - http://www.scopus.com/inward/record.url?scp=0028201345&partnerID=8YFLogxK

U2 - 10.1016/0898-6568(94)90062-0

DO - 10.1016/0898-6568(94)90062-0

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 8011430

AN - SCOPUS:0028201345

VL - 6

SP - 73

EP - 82

JO - Cellular Signalling

JF - Cellular Signalling

SN - 0898-6568

IS - 1

ER -

Enhanced transcription of the human α_2A-adrenergic receptor gene by cAMP: Evidence for multiple cAMP responsive sequences in the promoter region of this gene

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this