Enhanced transcription of the human α2A-adrenergic receptor gene by cAMP: Evidence for multiple cAMP responsive sequences in the promoter region of this gene

Lotan Shilo, Motoyoshi Sakaue, John M. Thomas, Mohan Philip, Brian B. Hoffman

Research output: Contribution to journalArticlepeer-review

Abstract

Expression of the human α2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5′-flanking sequences of the gene were placed in a promoterless vector with a chloramphenicol acetyltransferase (CAT) reporter gene, and cAMP-stimulated CAT activity was assayed in transfected JEG-3 placental carcinoma cells. Enhancer activity responsive to cAMP was located in DNA sequences both upstream and downstream from the endogenous promoter region. Within the upstream sequences there is a putative "core sequence" homologous to the eight base CRE consensus palindrome, but this region did not function independently as a CRE enhancer; additional upstream sequences were required to provide significant enhancer activity in response to cAMP. Regulation of expression of the α2A-adrenergic gene by cAMP is complex and involves multiple and likely novel DNA sequences.

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalCellular Signalling
Volume6
Issue number1
DOIs
StatePublished - Jan 1994
Externally publishedYes

Keywords

  • cAMP
  • enhancer
  • gene expression
  • transcription
  • transfection
  • α-adrenergic receptor

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